We narrowed to 18,981 results for: INO

1. pGEX6P1-hcGAS(157-522)

   Plasmid

   #108676

   Purpose

   For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

   Insert

   cGAS amino acid 157-522 (CGAS Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains amino acid 157-522

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-AcGFP-128-425-Akap9

   Plasmid

   #196871

   Purpose

   Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   AcGFP-Akap9 (128-425) (Akap9 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBactin-PACT-mOrange2-hCM1

   Plasmid

   #196868

   Purpose

   Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   PACT-mOrange2-hCM1 (Akap9 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. NES-EGFP-PH-BTKx2

   Plasmid

   #183652

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 2-174 and 2-170

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AIP(WT)-mChF-giantin

   Plasmid

   #61523

   Purpose

   Expression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Inserts

   FK506 binding protein 1A (FKBP1A Human)
   golgin B1 (GOLGB1 Human)
   

   Use

   Tags

   AIP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. NES-mCherry-PH-BTKx2

   Plasmid

   #183654

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-174 and 2-170

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcdna6.2- myc ULK1 k46I

   Plasmid

   #27630

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse myc ULK1 K46I (Myc Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Initial M from ULK1 removed. Silent mutation at A…

   Promoter

   Available since

   Feb. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pSAD-5PSD95-EGFP-SynPhRFP

   Plasmid

   #217979

   Purpose

   G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

   Depositor

   Edward Callaway

   Article

   Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

   Inserts

   5PSD95-EGFP (Dlg4 Rat)
   SynPhRFP (Syp Mouse)
   

   Use

   G-deleted rabies

   Tags

   EGFP and RFP

   Expression

   Mutation

   Promoter

   None

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Halo-Tag-128-425-Akap9

   Plasmid

   #196873

   Purpose

   Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Halo-Tag-Akap9 (128-425) (Akap9 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUltraHot-mCherry-BLM-WT

    Plasmid

    #206966

    Purpose

    expressing the protein BLM fused to mCherry in human cells

    Depositor

    Eran Hornstein

    Article

    Danino et al Nucleic Acids Res. 2023 Jul 28:gkad613.

    Insert

    Bloom Syndrome RecQ Like Helicase (BLM Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

    Plasmid

    #196885

    Purpose

    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-td-mCherry-Cdk5rap2-CTD

    Plasmid

    #196854

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcdna6.2- myc ULK1 4SA

    Plasmid

    #27631

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMK ENTR 1 CMV mAG beta-catenin

    Plasmid

    #206258

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mAG Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    Multimate/gateway entr 1

    Tags

    monomeric Azami Green

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-PIP5K3

    Plasmid

    #23806

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIP5K3 (PIKFYVE Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pBactin-AcGFP-Cdk5rap2-CTD

    Plasmid

    #196853

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCXIN- myc ULK1 wt

    Plasmid

    #27626

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQCXIN- myc ULK1 4SA

    Plasmid

    #27628

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA

    Plasmid

    #196896

    Purpose

    Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    N-Cadherin-2xHA (Cdh1 Mouse)
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQhsCDtRNA+

    Plasmid

    #86014

    Purpose

    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene

    Depositor

    Hong Li

    Article

    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

    Inserts

    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    

    Use

    Co-expression; purification

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    amino acids 1-401, amino acids 1-411, and amino a…

    Promoter

    LPP and Ptac

    Available since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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