We narrowed to 2,422 results for: NIG

1. pGL

   Plasmid

   #119952

   Purpose

   Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   gpps
   limS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminal truncation of 56 amino acid residues (…

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_hE2F7mg_PP7-tag

   Plasmid

   #73078

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   5'-PP7-tag RNA

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_hE2F7mg_2ntmut

   Plasmid

   #73074

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns, 2…

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pET41-FeoB-OL1del

   Plasmid

   #216741

   Purpose

   Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   feoB (PA4358 P. aeruginosa (Bacteria))
   

   Use

   Tags

   8x His

   Expression

   Bacterial

   Mutation

   Outer loop 1 (OL1) with the sequence of [INIGGALQ…

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFlare9A-Clip2E9 Mutant

   Plasmid

   #209575

   Purpose

   minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Clip2 (Clip2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

   Promoter

   CMV

   Available since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_hE2F7mg_6ntmut

   Plasmid

   #73075

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns wi…

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1_hE2F7mg_10ntmut

   Plasmid

   #73076

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns wi…

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUB6_EZH2

   Plasmid

   #84014

   Purpose

   EZH2 minigene to test splicing event

   Depositor

   Robert Bradley

   Article

   Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UB6

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSPL3-hRPH3A_c.444Gwt

   Plasmid

   #190476

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRP.CEP290 c.2991+1655A>G

    Plasmid

    #226556

    Purpose

    Minigene encoding the CEP290 c.2991+1655A>G mutation

    Depositor

    Dave Wee

    Article

    Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

    Insert

    CEP290 (CEP290 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    c.2991+1655A>G intronic mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSPL3-hRPH3A_c.1853Gmut

    Plasmid

    #190479

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.1853A>G

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_hE2F7mg_115mut

    Plasmid

    #73077

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with inserted SNORD115 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns, m…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRP.ABCA4 c.5461-10T

    Plasmid

    #226553

    Purpose

    Minigene encoding the ABCA4 c.5461-10T mutation

    Depositor

    Dave Wee

    Article

    Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

    Insert

    ABCA4 (ABCA4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.5461-10T intronic mutation

    Promoter

    CAG

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRP.ATM c.7865C>T

    Plasmid

    #226554

    Purpose

    Minigene encoding the ATM c.7865C>T mutation

    Depositor

    Dave Wee

    Article

    Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

    Insert

    ATM (ATM Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    c.7865C>T

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSPL3-hRPH3A_c.444Tmut

    Plasmid

    #190477

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.444G>T

    Promoter

    SV40

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSPL3-hRPH3A_c.1853Awt

    Plasmid

    #190478

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RG6-BAP1_E11

    Plasmid

    #154025

    Purpose

    Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 11

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
    

    Use

    Splicing reporter

    Tags

    DsRed1, FLAG, SV40 NLS, and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. RG6-BAP1_E7

    Plasmid

    #154023

    Purpose

    Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 7

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
    

    Use

    Splicing reporter

    Tags

    DsRed1, FLAG, SV40 NLS, and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQhsCDtRNA+

    Plasmid

    #86014

    Purpose

    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene

    Depositor

    Hong Li

    Article

    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

    Inserts

    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    

    Use

    Co-expression; purification

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    amino acids 1-401, amino acids 1-411, and amino a…

    Promoter

    LPP and Ptac

    Available since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. RG6-BAP1_E7-D192D

    Plasmid

    #154024

    Purpose

    Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
    

    Use

    Splicing reporter

    Tags

    DsRed1, FLAG, SV40 NLS, and eGFP

    Expression

    Mammalian

    Mutation

    BAP1 c.576C>T, p.D192D

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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