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pDMG10b

Plasmid

#33186

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

aex-4/tag-81 3'UTR (tag-81, aex-4 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-142 site: aCacTaC

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG10c

Plasmid

#33187

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

aex-4/tag-81 3'UTR (tag-81, aex-4 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

LTA site: atacGaT

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG1b

Plasmid

#33159

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

cog-1 3'UTR (cog-1 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-142 sites: aCacTaCa, aCacTaCa

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG4b

Plasmid

#33168

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

ptp-1 3'UTR (ptp-1 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-142 sites: CacTaCa, aCacTaCa

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCeSR beta deltaN

Plasmid

#13728

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (R186.3 Nematode)

Tags

6xHis

Expression

Bacterial

Mutation

expresses residues 38-240 of C. elegans SRβ with …

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

TEF1

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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wt Opa1

Plasmid

#204427

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Control-1

Plasmid

#204442

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L243A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_TAF3_2xPhd

Plasmid

#179399

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

TAF3_2xPhd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_2xChromo

Plasmid

#179396

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_2xChromo

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

G113V in flexible linker

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MBD1_MBD

Plasmid

#179404

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pKD001 pME minprom_EGFP

Plasmid

#195955

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann

Article

Prummel et al Nat Commun. 2019 Aug 26;10(1):3857. doi:

Insert

mouse beta-globin minimal promoter EGFP

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-acceptor-2xBbsI

Plasmid

#205633

Purpose

Cloning and U6-drive expression of ISDra2 TnpB reRNAs

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI

Expression

Mammalian

Promoter

U6

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJH2685

Plasmid

#191372

Purpose

Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Tetanus Toxin Light Chain

Tags

wCherry

Expression

Worm

Promoter

Punc-4

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-TBK1

Plasmid

#199205

Purpose

Used for the expression of TBK1 (SMC Internal No. 1996)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

Plasmid

#85583

Purpose

cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

Prab-3-GAL4-SK(DBD)-VP64

Expression

Worm

Promoter

Prab-3-GAL4-SK(DBD)-VP64

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159809

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN HA-NDP52

Plasmid

#199307

Purpose

For expression of the mammalian NDP52 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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