We narrowed to 6,263 results for: wus

1. mCherry-C1-TGAT

   Plasmid

   #84334

   Purpose

   Expresses TGAT tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

   Insert

   TGAT (TRIO Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-FLAG-CaMKIID

   Plasmid

   #186827

   Purpose

   Expresses FLAG-CaMKIID in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2d (Camk2d Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

   Plasmid

   #170229

   Purpose

   Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Q64L

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcCas12nHS_ABE_D3_empty

   Plasmid

   #203809

   Purpose

   Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   ABE_D3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132399

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-mouse vinculin full length (889)

   Plasmid

   #67935

   Purpose

   Mammalian expression of mouse vinculin with GFP fusion

   Depositor

   Alpha Yap

   Article

   Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

   Insert

   Vinculin (Vcl Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L159P in vinculin

   Promoter

   CMV

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a_SPI1_P2A_Hygro_Barcode

   Plasmid

   #120482

   Purpose

   Barcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

   Plasmid

   #176310

   Purpose

   Expresses CreN59-nMag-NLS with mCherry marker.

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   CreN59_nMag_NLS_P2A_mCherry_IRES_puroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1alpha

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. hCEP120-EGFP

   Plasmid

   #50382

   Purpose

   Over-expression of EGFP-tagged hCEP120

   Depositor

   Tang Tang

   Article

   Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

   Insert

   Human CEP120 (CEP120 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3 Flag MKK4

    Plasmid

    #14615

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    MKK4 (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a_Pro-IL-1α

    Plasmid

    #214341

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1A (IL1A Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK4_001

    Plasmid

    #123810

    Purpose

    Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Bgh pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK1_022

    Plasmid

    #123684

    Purpose

    Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS-A4 insulator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-CMV-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204339

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. MTK1_024

    Plasmid

    #123686

    Purpose

    Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector L2-C3 insulator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTK1_023

    Plasmid

    #123685

    Purpose

    Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector L1- B1 insulator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. (133) pcDNA3.1-Nup62-Flag-EPEA

    Plasmid

    #160744

    Purpose

    Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    Nup62-Flag-EPEA
    

    Use

    Tags

    EPEA and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMB-SaCas9

    Plasmid

    #105998

    Purpose

    SaCas9 expression vector

    Depositor

    Mike McManus

    Article

    Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

    Insert

    Cas9 nuclease
    

    Use

    CRISPR and Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    short EF1alpha

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK234_008

    Plasmid

    #123902

    Purpose

    Encodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    GFP dropout for multiTU destination vectors
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPBAD-yhjH

    Plasmid

    #111613

    Purpose

    Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

    Depositor

    Tim Tolker-Nielsen

    Article

    Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

    Insert

    yhjH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A52V

    Promoter

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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