We narrowed to 1,249 results for: vent

1. pCW-GemininΔDbox-HA-Puro

   Plasmid

   #193770

   Purpose

   Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   GemininΔDbox-HA (GMNN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Dbox removed (R23A, L26A). Codons switched at siR…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

   Plasmid

   #112762

   Purpose

   express GFP-4EBP1-Rheb15(CAAX mutation)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
   

   Use

   Lentiviral

   Tags

   Rheb15(CAAX mutant)

   Expression

   Mutation

   CAAX mutation to prevent lipid localization

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

   Plasmid

   #240228

   Purpose

   Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

   Depositor

   Norbert Perrimon, Justin Bosch

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

   Plasmid

   #240229

   Purpose

   Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

   Depositor

   Norbert Perrimon, Justin Bosch

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. An R57A, R59A CAAX pMT2

   Plasmid

   #206119

   Purpose

   Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R57A, R59A

   Promoter

   Ad MLP/TPL/SV40

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. An R57A, R59A HA CAAX pMT2

   Plasmid

   #206126

   Purpose

   Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R57A, R59A

   Promoter

   Ad MLP/TPL/SV40

   Available since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA tagged SphI ELL2 M133, 138, 186ILeu

   Plasmid

   #127277

   Purpose

   Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains HA tag near C-terminus

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 with Met 133, 138, 186 to Ileu (ELL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met 133, 138, 186 to Ileu; HA inserted at SphI af…

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCMVblast_SLC38A2_wt

   Plasmid

   #156180

   Purpose

   CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA se…

   Promoter

   CMV

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-APPP1-mGFP

   Plasmid

   #196705

   Purpose

   As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   mCherry and mEGFP

   Expression

   Mammalian

   Mutation

   Lys to Val substitution at position 612

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti.pFOXA2.GFP

    Plasmid

    #59404

    Purpose

    expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    400 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pFOXA2

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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