We narrowed to 23,941 results for: nes

1. pMaCTag-Z16

   Plasmid

   #120059

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Dronpa3
   

   Use

   Pcr template

   Tags

   Dronpa3

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-Z14

   Plasmid

   #120057

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mNeptune2.5
   

   Use

   Pcr template

   Tags

   mNeptune2.5

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Nano-lantern-Actin/pEGFP-C1

   Plasmid

   #51972

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   Actin

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pETM6-H9-mCherry

   Plasmid

   #66532

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter H9

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - H9

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Nano-lantern-peroxisome/pcDNA3

   Plasmid

   #51974

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomes

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   SKL

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Nano-lantern(Ca2+)_300/pRSETB

   Plasmid

   #51977

   Purpose

   bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-3GS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pMA4.1 (mouse integrin alpha 4 probe)

   Plasmid

   #14098

   Depositor

   Richard Hynes

   Article

   Yang et al Development. 1995 Feb . 121(2):549-60.

   Insert

   Mouse Alpha 4 Integrin (Itga4 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMaCTag-P04

   Plasmid

   #120015

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mTFP1
   

   Use

   Pcr template

   Tags

   mTFP1

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CT-Nano-lantern(ATP1)/pRSETB

   Plasmid

   #51990

   Purpose

   bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_ATP1
   

   Use

   Tags

   6xHis and chloroplast transit peptid

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

10. NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

    Plasmid

    #89994

    Purpose

    Donor template for generation of NGN3-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDGB3_omega1

    Plasmid

    #68238

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGB3_alpha2

    Plasmid

    #68229

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLIK TT 3xFLAG LacZ neo

    Plasmid

    #83105

    Purpose

    Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-Z18

    Plasmid

    #120061

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKeima8.5
    

    Use

    Pcr template

    Tags

    mKeima8.5

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Khc73-mCherry

    Plasmid

    #31752

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Khc73 (Khc-73 Fly)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Metallothionein

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Nano-lantern(Ca2+)_11/pRSETB

    Plasmid

    #51981

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM-4GS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pFAST-BAC-Cdc20wt (2193)

    Plasmid

    #39850

    Depositor

    Jonathon Pines

    Article

    Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

    Insert

    Cdc20 (CDC20 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

19. CT-Nano-lantern(ATP1)/pENTR1A

    Plasmid

    #51991

    Purpose

    Gateway entry vector for Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Entry vector

    Tags

    chloroplast transit peptid

    Expression

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pEIIIA

    Plasmid

    #14059

    Depositor

    Richard Hynes

    Article

    Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

    Insert

    Rat Fibronectin EIIIA (Fn1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EcoRI linkers added to TaqI to HinfI fragment

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pLX302-NIPP1-V5 puro

    Plasmid

    #87775

    Purpose

    Expresses V5 tagged human NIPP1

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    NIPP1 (PPP1R8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. nPEX-LV

    Plasmid

    #12119

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Matrix Metalloproteinase 2 (Mmp2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    noncatalytic fragment of MMP2 amplified via PCR, …

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

23. Nano-lantern(ATP1)/pRSETB

    Plasmid

    #51988

    Purpose

    bacterial expression of a Nano-lantern-based luminescent ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

24. Nano-lantern(Ca2+)_63/pcDNA3

    Plasmid

    #51986

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-4GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pEN_TTmiRc2_BirA

    Plasmid

    #136521

    Purpose

    Used as a donor vector to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA
    

    Use

    Entry vector for gateway cloning

    Tags

    Expression

    Mutation

    R118G (highly promiscuous form)

    Promoter

    Available since

    June 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK429

    Plasmid

    #155376

    Purpose

    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSLIK TT 3xFLAG LacZ hygro

    Plasmid

    #98395

    Purpose

    Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEN_TT miRc2 3xFLAG

    Plasmid

    #83274

    Purpose

    Empty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Type

    Empty backbone

    Use

    Entry vector for gateway cloning

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    TRE tight

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST-Hemmar-FLP(D)

    Plasmid

    #119895

    Purpose

    FLP(D) with a hsp70 promoter

    Depositor

    Rita Sousa-Nunes

    Article

    Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

    Insert

    FLP(D)
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    mutated form of FLP, which at position 5 contains…

    Promoter

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SEPLuc

    Plasmid

    #141102

    Purpose

    bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge