Skip to main content
Addgene
Showing: 4841 - 4880 of 8321 results

  1. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid
    #216520
    Purpose
    Expression of LegA7 ΔAnk290
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔAnk290
    Use
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 290-454
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216520-plasmid-map-sequence-id-430064

    Enlarge

  2. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid
    #216521
    Purpose
    Expression of LegA7 ΔNAnk264
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔNAnk264
    Use
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 264-361
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216521-plasmid-map-sequence-id-430065

    Enlarge

  3. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid
    #216522
    Purpose
    Expression of LegA7 ΔNAnk290
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔNAnk290
    Use
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 290-361
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216522-plasmid-map-sequence-id-430066

    Enlarge

  4. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-WT

    Plasmid
    #218834
    Purpose
    Expresses N-terminal BirA*-tagged human wild type TLK1 variant 5 for BioID experiments.
    Depositor
    Travis Stracker
    Insert
    TLK1 (TLK1 Human)
    Use
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218834-plasmid-map-sequence-id-434423

    Enlarge

  5. pRhaCAST

    Plasmid
    #211791
    Purpose
    pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter
    Depositor
    Silvia Cardona
    Insert
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Rhamnose-inducible
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211791-plasmid-map-sequence-id-418380

    Enlarge

  6. pET28-RPL3L-TwStrep

    Plasmid
    #208649
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949
    Depositor
    Quanfu Ma
    Insert
    human RPL3L (ENST00000268661) (RPL3L Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    contain rs753338989, rs746044959, rs752134949, pr…
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208649-plasmid-map-sequence-id-433652

    Enlarge

  7. pEB018

    Plasmid
    #215568
    Purpose
    Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus
    Depositor
    Robin Ohm
    Insert
    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
    Use
    Fungal homologous recombination plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    215568-plasmid-map-sequence-id-428736

    Enlarge

  8. pOET5 rota-VLP

    Plasmid
    #218147
    Purpose
    Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).
    Depositor
    Minna Hankaniemi
    Insert
    Rotavirus VP6
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218147-plasmid-map-sequence-id-433323

    Enlarge

  9. AAV-CAG-K-red-SPOTIT(R271F)

    Plasmid
    #191494
    Purpose
    Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-red-SPOTIT(R271F)
    Use
    AAV
    Tags
    Expression
    Mutation
    R271F in OR
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191494-plasmid-map-sequence-id-377895

    Enlarge

  10. AAV-CAG-M-red-SPOTIT2(R279F)

    Plasmid
    #191495
    Purpose
    Red fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-red-SPOTIT2(R279F)
    Use
    AAV
    Tags
    Expression
    Mutation
    R279F in OR
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191495-plasmid-map-sequence-id-377935

    Enlarge

  11. pET50_G495R MDA5

    Plasmid
    #216803
    Purpose
    Expresses a single point mutant of melanoma differentiation-associated protein 5
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 (IFIH1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G495R
    Promoter
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216803-plasmid-map-sequence-id-428833

    Enlarge

  12. pTRIPZ-NUDT17-C-HA-Puro

    Plasmid
    #195182
    Purpose
    Lentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.
    Depositor
    Stephanie Roessler
    Insert
    NUDT17 (NUDT17 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter and minimal CMV
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    195182-plasmid-map-sequence-id-423138

    Enlarge

  13. pDEST26-NAT1-C-HA

    Plasmid
    #195178
    Purpose
    Vector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
    Depositor
    Stephanie Roessler
    Insert
    NAT1 (NAT1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    195178-plasmid-map-sequence-id-423104

    Enlarge

  14. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

    Enlarge

  15. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

    Enlarge

  16. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

    Enlarge

  17. pCHA_SBV_NSs

    Plasmid
    #208938
    Purpose
    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 47 bp at 5' end and 510 bp from 3&#x…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208938-plasmid-map-sequence-id-419026

    Enlarge

  18. pET28-Flag-RBM24-95~236

    Plasmid
    #208634
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 95~236, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208634-plasmid-map-sequence-id-418349

    Enlarge

  19. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

    Enlarge

  20. pAGW-Fray

    Plasmid
    #203979
    Purpose
    GFP-Fray under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Frayed (fray Fly)
    Use
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203979-plasmid-map-sequence-id-403220

    Enlarge

  21. pAMW-WnkAAxV

    Plasmid
    #203980
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Use
    Tags
    6xMyc
    Expression
    Insect
    Mutation
    RFxV mutated to AAxV
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203980-plasmid-map-sequence-id-403221

    Enlarge

  22. pAG426GAL-FES

    Plasmid
    #203501
    Purpose
    Expresses human FES kinase from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    FES (FES Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    203501-plasmid-map-sequence-id-402839

    Enlarge

  23. pAG416GAL10-c-Src

    Plasmid
    #203462
    Purpose
    Expresses human c-Src kinase from a GAL10 promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    c-Src (CSK Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203462-plasmid-map-sequence-id-402862

    Enlarge

  24. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

    Enlarge

  25. pGB-06-07-Vps34

    Plasmid
    #187989
    Purpose
    Plasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323
    Depositor
    Sascha Martens
    Insert
    Vps34 (PIK3C3 Human)
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187989-plasmid-map-sequence-id-374438

    Enlarge

  26. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

    Enlarge

  27. p2151 pHAGE-hSPC-TFEB-TagBFP-W

    Plasmid
    #188722
    Purpose
    Lentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion protein
    Depositor
    Darrell Kotton
    Insert
    TFEB (TFEB Human)
    Use
    Lentiviral
    Tags
    tagBFP
    Expression
    Mutation
    Promoter
    hSP-C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188722-plasmid-map-sequence-id-372785

    Enlarge

  28. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid
    #132622
    Purpose
    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges
    Depositor
    Constance Ciaudo
    Insert
    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    132622-plasmid-map-sequence-id-259572

    Enlarge

  29. mT2A-TagRFP-T-linker-dre-PBD*-FNF

    Plasmid
    #182342
    Purpose
    Recombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-TagRFP-T-linker-dre-PBD*-FNF
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182342-plasmid-map-sequence-id-358194

    Enlarge

  32. CNN3A-c007

    Plasmid
    #175457
    Purpose
    Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.
    Depositor
    Opher Gileadi
    Insert
    CNN3 (CNN3 Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175457-plasmid-map-sequence-id-353125

    Enlarge

  33. pLV V5-Eva1b

    Plasmid
    #175158
    Purpose
    Lentiviral expression of V5-tagged mouse Eva1b
    Depositor
    Larry Gerace
    Insert
    Eva1b (Eva1b Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175158-plasmid-map-sequence-id-352269

    Enlarge

  34. pLV Cenpv-V5

    Plasmid
    #175125
    Purpose
    Lentiviral expression of mouse Cenpv-V5
    Depositor
    Larry Gerace
    Insert
    Cenpv (Cenpv Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175125-plasmid-map-sequence-id-351968

    Enlarge

  35. pX330.orup

    Plasmid
    #110404
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110404-plasmid-map-sequence-id-307700

    Enlarge

  36. Clim Act MCH (CAM)

    Plasmid
    #104443
    Purpose
    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    actin
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104443-plasmid-map-sequence-id-200869

    Enlarge

  37. Clim EF1 MCH (CEM)

    Plasmid
    #104444
    Purpose
    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104444-plasmid-map-sequence-id-200864

    Enlarge

  38. Clim Tub MCH (CTM)

    Plasmid
    #104445
    Purpose
    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    tubulin promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104445-plasmid-map-sequence-id-200865

    Enlarge

  39. Lentiviral human Citron shRNA 1 GFP

    Plasmid
    #155286
    Purpose
    Lentiviral expression of human CIT shRNA, GFP expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Citron (CIT Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155286-plasmid-map-sequence-id-310000

    Enlarge

  40. pHAGE-hSPC-TFEB(delta30)eGFP-W

    Plasmid
    #126693
    Purpose
    Lineage specific over expression of a mutated TFEB in lung alveolar epithelial cells, where TFEB is missing the first 30 amino acids of the sequence.
    Depositor
    Darrell Kotton
    Insert
    TFEB(delta30) (TFEB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deleted amino acids 1-30, H32D, Deleted Stop Codo…
    Promoter
    hSPC
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126693-plasmid-map-sequence-id-245693

    Enlarge

Showing: 4841 - 4880 of 8321 results