We narrowed to 15,732 results for: LEA

1. C113m

   Plasmid

   #121012

   Purpose

   MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. C95m

   Plasmid

   #121001

   Purpose

   MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   T7 RNA polymerase (Andy please check!)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 371-A

   Plasmid

   #97026

   Purpose

   yORF

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ (M)-HT-NLS

   Plasmid

   #82518

   Purpose

   Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.

   Depositor

   Xiaojun Ren

   Insert

   Nuclear localizaition sequence
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTagBFP-Nucleus-7

   Plasmid

   #55265

   Purpose

   Localization: Nuclear Localization, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Insert

   SV40 T-Antigen Nuclear Localization Signal (NLS)
   

   Tags

   mTagBFP-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3-TopBP1-mCer

   Plasmid

   #211414

   Purpose

   mammalian expression of human TopBP1 C-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   TopBP1 (TOPBP1 Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpen-K12polLF

   Plasmid

   #165511

   Purpose

   DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   DNA Polymerase I, Large (Klenow) Fragment
   

   Use

   Synthetic Biology

   Available Since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.1-aASIC1

   Plasmid

   #32795

   Depositor

   Cecelia Canessa

   Insert

   ASIC1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAIP4_3A7L

   Plasmid

   #234182

   Purpose

   Heterologous protein expression of glycine cleavage system H protein in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3A7L (GCV3 Escherichia coli K-12)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3-RecQL4-mCer

    Plasmid

    #211402

    Purpose

    mammalian expression of human RecQL4 C-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    RecQL4 (RECQL4 Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 371-U

    Plasmid

    #97036

    Purpose

    MYFQSNA-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    MYFQSNA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid

    #52421

    Purpose

    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

    Depositor

    Bertrand Collet

    Insert

    Fusion NLS-EGFP-P2A-Cherry-CAAX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. EBFP2-Nucleus-7

    Plasmid

    #55249

    Purpose

    Localization: Nuclear Localization, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    EBFP2-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pACYC-LIC+

    Plasmid

    #62312

    Purpose

    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and thrombin protease cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

15. mEmerald-Nucleus-7

    Plasmid

    #54206

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    mEmerald-Nucleus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. U25m

    Plasmid

    #121027

    Purpose

    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    RiboJ + UTR1
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMJO-7

    Plasmid

    #31992

    Depositor

    Mark Osborn

    Article

    Osborn et al Hum Gene Ther. 2011 Jun 13.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pChillyBoys_gen1

    Plasmid

    #228495

    Purpose

    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)

    Depositor

    Richard Baker

    Inserts

    groEL
    groES
    

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCer-C3-Plk1

    Plasmid

    #68132

    Purpose

    Expression of Cerulean-tagged Plk1 in mammalian cells

    Depositor

    Catherine Lindon

    Insert

    Polo-like kinase 1 (PLK1 Human)
    

    Tags

    Cerulean

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACYC-GST

    Plasmid

    #62329

    Purpose

    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    Glutathione S-transferase (GST) tag and TEV prote…

    Expression

    Bacterial

    Promoter

    T7-lac0

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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