Addgene

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RcSxph F784S

Plasmid

#194033

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

F784S

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph F784Y

Plasmid

#194034

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

F784Y

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph D785A

Plasmid

#194035

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

D785A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph D785N

Plasmid

#194036

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

D785N

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph Q787A

Plasmid

#194037

Purpose

Protein expression and characterizatio

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Q787A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph Q787E

Plasmid

#194038

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Q787E

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph K789A

Plasmid

#194039

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

K789A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph D794A

Plasmid

#194040

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

D794A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph D794E

Plasmid

#194041

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

D794E

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph D794N

Plasmid

#194042

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

D794N

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph Y795A

Plasmid

#194043

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Y795A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RcSxph Y558A/I782A

Plasmid

#194044

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Y558A/I782A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FAK Fus

Plasmid

#186145

Purpose

For expression of recombinant FAK using baculovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Use

Tags

His6

Expression

Insect

Mutation

C-terminal linker swapped with FUS IDR

Promoter

polyhedrin promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lck

Plasmid

#186130

Purpose

For expression of recombinant Lck in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

Lck (Lck Mouse)

Use

Tags

His6

Expression

Insect

Mutation

A1687T

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FAK Pyk2

Plasmid

#186146

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Use

Tags

His6

Expression

Insect

Mutation

C-terminal linker swapped with Pyk2 linker

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Cquin_728

Plasmid

#176656

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Cquin_801

Plasmid

#176657

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Cquin_801

Plasmid

#176671

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Cquin_728

Plasmid

#176670

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Mdn1-deltaC

Plasmid

#171603

Purpose

Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Use

Tags

His6 tag

Expression

Insect

Mutation

C-terminal deletion; a.a. 1-3911 only

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge