We narrowed to 23,988 results for: nes

1. His-PHD2-181-426

   Plasmid

   #223551

   Purpose

   His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-180.

   Promoter

   trc

   Available since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSS02:cyt-Peredox-mCherry_DS

   Plasmid

   #161747

   Purpose

   cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

   Depositor

   Markus Schwarzländer

   Article

   Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

   Insert

   cyt-Peredox-mCherry_DS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   amino acid substitution D115S in first T-Rex doma…

   Promoter

   Ubiquitin 10

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOmnibac_ZZ_linker_K560_GFP_SNAP

   Plasmid

   #159720

   Purpose

   Expresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   KIF5B (only amino acids 1-560), K560 (KIF5B Human)
   

   Use

   Tags

   GFP and SNAP tags and ZZ affinity tag

   Expression

   Insect

   Mutation

   Only amino acids 1-560

   Promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

   Plasmid

   #135812

   Purpose

   This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

   Depositor

   Nadia Mercader Huber

   Article

   Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

   Insert

   TagRFP
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p1.1-mCherry

   Plasmid

   #162772

   Purpose

   Fluorescent reporter for CHO expression studies

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

   Insert

   red fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p1.2-GS-eGFP

   Plasmid

   #162771

   Purpose

   Fluorescent reporter for CHO expression studies

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

   Insert

   enhanced green fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1

   Plasmid

   #175814

   Purpose

   pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller, Mark Banfield

   Article

   Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

   Insert

   IMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
   

   Use

   Pentr plasmid for gateway cloning

   Tags

   none

   Expression

   Mutation

   Promoter

   none

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

   Plasmid

   #171101

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Olig2 S. pyogenes, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. RCAS-Neu HA SIINFEKL

   Plasmid

   #125832

   Purpose

   Retroviral expression of rat Neu cDNA

   Depositor

   Yi Li

   Article

   Du et al Proc Natl Acad Sci U S A. 2006 Nov 14;10

   Insert

   Neu (Erbb2 Rat)
   

   Use

   Retroviral

   Tags

   2x HA and SIINFEKL

   Expression

   Mammalian

   Mutation

   Val to Glu point mutation of codon 664 and trunca…

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

    Plasmid

    #122959

    Purpose

    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Expression

    Mutation

    deletion of C terminus from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

    Plasmid

    #122956

    Purpose

    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    deletion of C terminus from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-nSpCas9-TadA8.20m

    Plasmid

    #209045

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TadA8.20m-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Donor_YTHDF2_KO_Neo

    Plasmid

    #186672

    Purpose

    Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Neomycin (YTHDF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Donor_ANKRD1_KO_Hygro

    Plasmid

    #186668

    Purpose

    Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    ANKRD1 KO Hygromycin (ANKRD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOmnibac_ZZ_linker_K490_GFP_SNAP

    Plasmid

    #159721

    Purpose

    Expresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    KIF5B (only amino acids 1-490), K490 (KIF5B Human)
    

    Use

    Tags

    GFP and SNAP tags and ZZ affinity tag

    Expression

    Insect

    Mutation

    Only amino acids (1-490)

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. DOT1L-CC3

    Plasmid

    #111754

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #3 (residues 613-661)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. DOT1L-CC1

    Plasmid

    #111756

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    contains coiled coil #1 (residues 506-555)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. DOT1L-CC2

    Plasmid

    #111755

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #2 (residues 560-608)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pdCas9-DNMT3A-PuroR

    Plasmid

    #71667

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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