Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 8,780 results for: construct

Clear keywords
Showing: 4881 - 4920 of 8780 results

  1. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

    Enlarge

  2. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196078
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196078-plasmid-map-sequence-id-388989

    Enlarge

  3. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196076
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196076-plasmid-map-sequence-id-388979

    Enlarge

  4. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

    Enlarge

  6. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

    Plasmid
    #219701
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219701-plasmid-map-sequence-id-435731

    Enlarge

  7. pPB-PGK-mCherry.OVA(323-339)

    Plasmid
    #190005
    Purpose
    Piggybac construct for constitutive expression of mCherry and Chicken Ovalbumin OVA323-339 peptide fusion
    Depositor
    Siyuan Zhang
    Insert
    mCherry.OVA(323-339)
    Use
    Synthetic Biology; Piggybac
    Tags
    OVA(323-339)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    190005-plasmid-map-sequence-id-382848

    Enlarge

  8. KARS_pNIC-Bio3

    Plasmid
    #153051
    Purpose
    Expression construct for producing Avi tagged Lysyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    KARS (KARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153051-plasmid-map-sequence-id-304466

    Enlarge

  10. pTrcHisA-PPARalpha(aa101-468)

    Plasmid
    #13574
    Depositor
    Martin Scanlon
    Insert
    human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
    Tags
    x6His N-terminal, enterokinase cleavage site
    Expression
    Bacterial
    Mutation
    deleted residues 1-100.
    Available Since
    March 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    13574-plasmid-map-sequence-id-142611

    Enlarge

  11. MSCV-mMDM2-IRES-GFP

    Plasmid
    #66954
    Purpose
    retroviral construct for expression of mouse Mdm2 cloned into the EcoRI site
    Depositor
    Martine Roussel
    Insert
    Mdm2 (Mdm2 Mouse)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Promoter
    MSCV
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66954-plasmid-map-sequence-id-173086

    Enlarge

  13. pUPD2 PhyB (GB2721)

    Plasmid
    #160603
    Purpose
    chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.
    Depositor
    Diego Orzaez
    Insert
    PhyB
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160603-plasmid-map-sequence-id-322052

    Enlarge

  14. HARS_pNIC-Bio3

    Plasmid
    #153050
    Purpose
    Expression construct for producing Avi tagged histidyl tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    HARS (HARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153050-plasmid-map-sequence-id-304465

    Enlarge

  17. Tet-O-FUW-NR2F6-PURO

    Plasmid
    #251733
    Purpose
    Overexpression construct expressing NR2F6-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    NR2F6 (NR2F6 Human)
    Use
    Lentiviral
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251733-plasmid-map-sequence-id-501477

    Enlarge

  18. pMbd3-GGSG-AID-3xF_PP

    Plasmid
    #235507
    Purpose
    Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, roxed Pgk-Puro
    Depositor
    Brian Hendrich
    Insert
    Mbd3 (Mbd3 Mouse)
    Use
    Mouse Targeting
    Tags
    AID, 3xFLAG
    Mutation
    None
    Promoter
    PGK
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235507-plasmid-map-sequence-id-474013

    Enlarge

  19. pChd8_GGSG_Fkbp_2xHA_BB

    Plasmid
    #235524
    Purpose
    Targeting construct: Chd8-FKBP-HA2 with Roxed Blasto cassette
    Depositor
    Brian Hendrich
    Insert
    Chd8 (Chd8 Mouse)
    Use
    Mouse Targeting
    Tags
    FKBP, 2xHA
    Mutation
    None
    Promoter
    beta actin
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235524-plasmid-map-sequence-id-473993

    Enlarge

  20. pMX-Flag-GFP_CBLN3

    Plasmid
    #227950
    Purpose
    Control plasmid. Important note: Construct with N-terminally tagged GFP which might mask the signal sequence for proper localization of the protein
    Depositor
    Alessandro Ori
    Insert
    CBLN3 (CBLN3 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Available Since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227950-plasmid-map-sequence-id-455399

    Enlarge

  21. pT2/shαSMA/GFP4_Seq1/clone1

    Plasmid
    #206138
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    alpha-SMA (Acta2 Mouse)
    Expression
    Mammalian
    Available Since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206138-plasmid-map-sequence-id-410128

    Enlarge

  22. pCASCADE-gltA1-gltA2-udhA-zwf

    Plasmid
    #87152
    Purpose
    gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-zwf guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87152-plasmid-map-sequence-id-174941

    Enlarge

  23. pCASCADE-fabI-gltA1-gltA2-gapAP1

    Plasmid
    #87147
    Purpose
    fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87147-plasmid-map-sequence-id-174935

    Enlarge

  25. STING-V1

    Plasmid
    #124262
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus1
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124262-plasmid-map-sequence-id-241476

    Enlarge

  27. STING-V2

    Plasmid
    #124264
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus2
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124264-plasmid-map-sequence-id-241461

    Enlarge

  28. V1-STING

    Plasmid
    #124263
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus1
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124263-plasmid-map-sequence-id-241474

    Enlarge

  29. V2-STING

    Plasmid
    #124265
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus2
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124265-plasmid-map-sequence-id-241431

    Enlarge

  30. mu-FLT3Lv1-pTRIP

    Plasmid
    #163519
    Purpose
    Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells
    Depositor
    Thomas Weber
    Insert
    murine-FLT3L variant 1 (Flt3l Mouse)
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163519-plasmid-map-sequence-id-321571

    Enlarge

  33. pSLIK CXADR-V5 hygro

    Plasmid
    #220362
    Purpose
    Lentiviral expression vector for an inducible V5-tagged CXADR construct
    Depositor
    Kevin Janes
    Insert
    CXADR (CXADR Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220362-plasmid-map-sequence-id-436884

    Enlarge

  35. MARS_pNIC-CTB10H

    Plasmid
    #153053
    Purpose
    Expression construct for producing Avi tagged Methionyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    MARS (MARS1 Human)
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153053-plasmid-map-sequence-id-304471

    Enlarge

  37. pEB018

    Plasmid
    #215568
    Purpose
    Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus
    Depositor
    Robin Ohm
    Insert
    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
    Use
    Fungal homologous recombination plasmid
    Available Since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    215568-plasmid-map-sequence-id-428736

    Enlarge

  38. pAAV2-CAGIG-WGA-mCherry

    Plasmid
    #224119
    Purpose
    To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.
    Depositor
    Pantelis Tsoulfas
    Insert
    WGA-mCherry
    Use
    AAV
    Tags
    WGA and mCherry
    Expression
    Mammalian
    Mutation
    All mutations related to the WGA in this construc…
    Promoter
    CAGIG
    Available Since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    224119-plasmid-map-sequence-id-446520

    Enlarge

  39. hSyn-FAS-DO-ReaChR-mCitrine-WPRE

    Plasmid
    #64035
    Purpose
    Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs
    Depositor
    Karel Svoboda
    Insert
    hSyn-FAS-DO-ReaChR-mCitrine-WPRE
    Use
    AAV
    Tags
    ReaChR tagged with mCitrine
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 4881 - 4920 of 8780 results