We narrowed to 2,701 results for: LRE

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CDK20 gRNA (BRDN0001144911)

Plasmid

#75706

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCW57-mCherry-2A-BRD4 Iso AdelCTD

Plasmid

#137722

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Truncated at amino acid 1328

Promoter

Tight TRE promoter

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57-mCherry-2A-BRD4 Iso CdelET

Plasmid

#137723

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Deleted amino acids 600-682

Promoter

Tight TRE promoter

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PB-tetO-NB

Plasmid

#197092

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mCherry-53BP1

Plasmid

#127658

Purpose

Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

Plasmid

#67977

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

Plasmid

#67974

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2ABFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-Cas9Bsd-W

Plasmid

#68343

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 ORF3a C-V5-tag

Plasmid

#179980

Purpose

Mammalian expression vector for SARS-CoV-2 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-2 ORF3a (ORF3a Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PAX3::FOXO1ΔHD-2A-sfGFP

Plasmid

#240099

Purpose

human PAX3::FOXO1 with homeobox domain deletion in -2A-sfGFP backbone (Plasmid# 240099)

Depositor

Genevieve Kendall

Article

Kucinski et al Cell Rep. 2025 Jul 22;44(7):115923. doi:

Insert

PAX3::FOXO1-minusHD (FOXO1, PAX3 Human)

Expression

Mammalian

Mutation

removed amino acids 218-278 for the homeobox DNA …

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-66Q-myc-WPRE

Plasmid

#107934

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)

Use

AAV

Promoter

hSynapsin

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Bsd-CMV>hCDK11A

Plasmid

#239212

Purpose

Expresses CDK11A in mammalian cell lines

Depositor

Jason Sheltzer

Article

Julian et al bioRxiv 2025.08.03.668359; doi: https://

Insert

cyclin dependent kinase 11A (CDK11A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-RNA-pCAG-bpNLS-miCBE-SV40NLS-bGH polyA-pCMV-mCherry-AmpR

Plasmid

#248061

Purpose

Expression vector for expression of miCBE-ωRNA v2 driven by chicken β-actin promoter and mCherry driven by CMV promoter.

Depositor

Chunlong Xu

Article

Guo et al Cell Rep. 2024 Nov 26;43(11):114973. doi

Insert

pU6-RNA-pCAG-bpNLS-miCBE-SV40NLS-bGH polyA-pCMV-mCherry-AmpR

Use

CRISPR

Tags

flag

Expression

Mammalian

Mutation

none

Promoter

CAG, U6

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-EGFP-PARP1

Plasmid

#176146

Purpose

EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCSC-ISL1-T2A-LHX3

Plasmid

#90215

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

ISL1-T2A-LHX3 (ISL1, LHX3 Human)

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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