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p413_Csy4NLS

Plasmid

#103139

Purpose

Expresses Csy4-NLS under TEF promoter

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

Csy4

Tags

NLS

Expression

Yeast

Promoter

TEF

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRF-PsrA

Plasmid

#49368

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

PsrA repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGBScomp High

Plasmid

#223202

Purpose

Group B Streptococcus High Expression Modular Complementation Vector

Depositor

Thomas Hooven

Article

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

Complementation vector

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGBScomp Low

Plasmid

#223203

Purpose

Group B Streptococcus Low Expression Modular Complementation Vector

Depositor

Thomas Hooven

Article

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

Complementation vector

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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LT1_33_pTEFmut8-mCherry

Plasmid

#177706

Purpose

Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEFmut8-mCherry

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

Plasmid

#66708

Purpose

expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

Depositor

Charles Gersbach

Insert

S.Pyogenes VP64-dCas9-VP64

Use

Lentiviral and Synthetic Biology

Tags

VP64

Mutation

D10A and H840A

Promoter

hUbC

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn-pOUT

Plasmid

#213905

Purpose

Mariner transposon with outward-facing pJ23104 promoter

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and outward-facine pJ23104 promoter

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBHM2617

Plasmid

#234496

Purpose

NATR-CnU6-empty sgRNA

Depositor

Hiten Madhani

Article

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

NAT-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHelper_AraC/pBad-Tnpase (camR)

Plasmid

#213909

Purpose

Arabinose-inducible himar1C9 transposase expression - cam resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

AraC/pBad ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRF-BetI

Plasmid

#49357

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

BetI repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRVV669

Plasmid

#108283

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAC575

Plasmid

#127976

Purpose

AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

ble (bleomycin resistance marker)

Use

Fungal expression

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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p2attPC-FO

Plasmid

#51551

Purpose

DICE donor plasmid with the FOXA2 and OTX2 transcription factors.

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Inserts

FOXA2
OTX2

Expression

Mammalian

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pEntr_PE2

Plasmid

#149548

Purpose

Gateway entry clone with PE2 enzyme (with stop codon)

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR; Gateway shuttle vector

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2-EM7neoPheS

Plasmid

#112923

Purpose

piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

neoPheS

Use

Piggybac transposon gateway destination vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p3xP3-EGFP.vas-int.NLS

Plasmid

#60948

Purpose

Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

vas-phiC31 integrase.NLS

Use

Rmce

Promoter

vasa

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only
pECO300

Plasmid

#169766

Purpose

Express SpCas9 and Kanamycin resistance gene

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Plant binary vector

Expression

Plant

Promoter

35S, AtU6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRF-McbR

Plasmid

#49365

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

McbR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAd5-B3/4

Plasmid

#175785

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B3 and pAd5-B4.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Adenovirus 5 genomic region 10862-18018

Use

Adenoviral and Synthetic Biology

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA11 1397N

Plasmid

#221935

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA11 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: S1330I, A1341V, N1342S, E1370K, T1380I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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