We narrowed to 11,959 results for: pcDNA

1. pcDNA3.1 3xF-NHP2

   Plasmid

   #126871

   Purpose

   Expresses 3xFlag NHP2

   Depositor

   Kathleen Collins

   Article

   Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

   Insert

   NHP2 (NHP2 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_C-6His-Stx17

   Plasmid

   #112259

   Purpose

   C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)

   Depositor

   Madhulika Tripathi

   Insert

   syntaxin 17 (Stx17 Mouse)
   

   Use

   Tags

   6His Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-DAKAP-μDpTT

   Plasmid

   #182324

   Purpose

   Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   DAKAP-μDronpa-TagRFP-T
   

   Use

   Tags

   N-terminal 30 amino acids from DAKAP1

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Dest53-SHP-1 WT

   Plasmid

   #21791

   Depositor

   Stephen Shaw

   Article

   Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

   Insert

   SHP-1 (PTPN6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5/FRT/TO V5 HSPA14

   Plasmid

   #19515

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 14 (HSPA14 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4-Beclin1 1-242

   Plasmid

   #24391

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-242.

   Promoter

   Available since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-Myc-CyclinD1(WT)

   Plasmid

   #122300

   Purpose

   expresses Cyclin D1 protein with a Myc tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Casp9 C287A

   Plasmid

   #11819

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

   Insert

   Caspase 9 catalytically incompetent mutant (CASP9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C287A, catalytically incompetent mutant.

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-RAB-EKARev(T/A)

   Plasmid

   #118475

   Purpose

   Negative-control mutant for RAB-EKARev biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-EKARev(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-ExRai-AktAR(T/A)

    Plasmid

    #118463

    Purpose

    Negative-control mutant for ExRai-AktAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3puro-mGFP-TOP3B

    Plasmid

    #225470

    Purpose

    Express mEGFP-fusion protein of TOP3B

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    TOP3B (TOP3B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-FRT-TO-FH-Nsp16

    Plasmid

    #157724

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp16 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA3-delta133HtrA-EGFP

    Plasmid

    #14124

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the first 133 amino-acids

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-TIE-V5/HIS

    Plasmid

    #205612

    Purpose

    Expression of human TIE receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TIE receptor tyrosine kinase (TIE1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3 Flag Jnk1a2

    Plasmid

    #13751

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk1a2 (MAPK8 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1(+)-OTI-TCRB

    Plasmid

    #131036

    Purpose

    Expresses OT-I TCRB

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Mito-CaNAR2

    Plasmid

    #64731

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 30 amino acids from DAKAP1,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3/Myc-DNMT3B2

    Plasmid

    #36942

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B2 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA4.TO-ORF57-2xCSTREP

    Plasmid

    #136217

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF57
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcdna3 Flag Jip1b

    Plasmid

    #52123

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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