We narrowed to 17,732 results for: ERG

1. L2092-GPA-FullBRET-Retrovirus-TD142

   Plasmid

   #222877

   Purpose

   Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-NanoLuc-CyOFP1
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

   Plasmid

   #127362

   Purpose

   gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2,0_3M5M_eGFP

   Plasmid

   #135474

   Purpose

   Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   Marburg virus minigenome, eGFP reporter
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   T7

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSF107mT-GATEWAY-3'-Myc tag

   Plasmid

   #67617

   Purpose

   A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

   Depositor

   Todd Stukenberg

   Article

   Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

   Type

   Empty backbone

   Use

   In vitro transcription or translation off of an s…

   Tags

   C-terminal Myc

   Expression

   Mammalian

   Promoter

   CMV and SP6

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115267

   Purpose

   Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   LbuCas13a
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ heiCas9

   Plasmid

   #186929

   Purpose

   For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

   Depositor

   Joachim Wittbrodt

   Article

   Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

   Insert

   myc-flexible linker-oNLS-Cas9-oNLS
   

   Use

   CRISPR

   Tags

   myc-flexible linker-optimizedNLS and optimizedNLS

   Expression

   Mammalian

   Promoter

   sp6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP1337

   Plasmid

   #119270

   Purpose

   for cloning new sgRNAs

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. T7-Elongin C-pcDNA3

   Plasmid

   #19998

   Depositor

   William Kaelin

   Article

   Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

   Insert

   Elongin C (ELOC Human)
   

   Tags

   T7-tag

   Expression

   Mammalian

   Available Since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. L2125-FRB-GPA-NLuc-CyOFP1

   Plasmid

   #222885

   Purpose

   Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc-10GS-CyOFP1
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2,0_3M5M_luciferase

    Plasmid

    #135475

    Purpose

    Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, luciferase reporter
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-mDyn1 pcDNA3

    Plasmid

    #36263

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 1 (Dnm1 Mouse)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pORTMAGE-1

    Plasmid

    #72680

    Purpose

    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    lower strength RBS before mutL E32K allele than i…

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pSL0284 (pQCascade_entry)

    Plasmid

    #130635

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDF0317 HvsCas7-11 mammalian expression

    Plasmid

    #172511

    Purpose

    Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    HvsCas7-11 mammalian expression
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-FOXO3a TM-ER

    Plasmid

    #8353

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, mouse estrogen receptor (G525…

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pENTR_L1-YFP-miniTurbo-L2

    Plasmid

    #127352

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR_L1-Turbo-YFP-NLS-STOP-L2

    Plasmid

    #127361

    Purpose

    gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. E42_gRunx1_gRNA3_dTet_NGFR

    Plasmid

    #189803

    Purpose

    Retroviral delivery of guide RNA against mouse Runx1

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx1_gRNA3 (Runx1 Mouse)
    

    Use

    Retroviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

    Plasmid

    #194231

    Purpose

    Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a-LCB1-PDGFR TMD-T2A-mCherry
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-FOXO3a TM-ER-delta DB

    Plasmid

    #8354

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, ER (G525R), delta DB (deletio…

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only