We narrowed to 3,022 results for: ER

1. pRSFDuet-FBXL5-SKP1

   Plasmid

   #226620

   Purpose

   Co-expression of FBXL5-SKP1 complex in E.coli

   Depositor

   Ning Zheng

   Article

   Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

   Inserts

   FBXL5 (FBXL5 Human)
   SKP1 (SKP1 Human)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   Amino acids 1-198 was deleted, and 420-596 was re…

   Promoter

   T7

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104493

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV Retrograde and AAV1

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104497

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPRv2-sgCD20

   Plasmid

   #209749

   Purpose

   Lentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG426GAL-SARS-CoV-2_PLP

   Plasmid

   #203509

   Purpose

   Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   SARS-CoV-2 PLP (ORF1ab )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TMEM216-mCherry

   Plasmid

   #41633

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 216 (TMEM216 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

   Plasmid

   #197505

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

   Promoter

   CMV and T7

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TMEM138-mCherry

   Plasmid

   #41630

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 138 (TMEM138 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA TMEM 138-flag

   Plasmid

   #41628

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 138 (TMEM138 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

    Plasmid

    #236241

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV1.2 (CACNA1C Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

    Plasmid

    #236239

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV2.1 (CACNA1A Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV hSyn INTRON HaloTag-Sec61b WPRE

    Plasmid

    #236229

    Purpose

    AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    HaloTag-Sec61b (SEC61B Synthetic, Human)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid

    #236245

    Purpose

    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    U6 and human Synapsin1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

    Plasmid

    #236240

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV2.2 (CACNA1B Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV hSyn INTRON mEmerald-Sec61b WPRE

    Plasmid

    #236228

    Purpose

    AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mEmerald-Sec61b (SEC61B Synthetic, Human)
    

    Use

    AAV

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-KLF2-T2A-mCHERRY

    Plasmid

    #224295

    Purpose

    Expresses mouse KLF2 and mCherry under EF1a promoter

    Depositor

    Nikhil Joshi

    Article

    Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

    Insert

    KLF2 T2A mCherry (Klf2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a-TBET-T2A-mCHERRY

    Plasmid

    #224296

    Purpose

    Expresses mouse TBET and mCherry under EF1a promoter

    Depositor

    Nikhil Joshi

    Article

    Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

    Insert

    TBET T2A mCherry (Tbx21 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid

    #222500

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E and E814…

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRE3GV-SpdCas9-Dnmt3A/3L-V1

    Plasmid

    #222503

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRE3GV-SpdCas9-Dnmt3A/3L-V2

    Plasmid

    #222505

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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