We narrowed to 18,217 results for: Met;

1. pEB1-sfGFP

   Plasmid

   #103983

   Purpose

   Plasmid encoding sfGFP

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   sfGFP
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S30R, Y39N, …

   Promoter

   proC

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5HT6-CFP-Venus(H148G)

   Plasmid

   #47501

   Purpose

   Fluorescent reporter for pH inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   Venus(H148G)
   

   Use

   Tags

   5HT6 and CFP

   Expression

   Mammalian

   Mutation

   H148G mutation

   Promoter

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

3. dBrainbow

   Plasmid

   #29327

   Depositor

   Julie Simpson

   Article

   Hampel et al Nat Methods. 2011 Mar;8(3):253-9. doi: 1

   Insert

   UAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. GatA_pET29b

   Plasmid

   #215406

   Purpose

   Expression construct for GatA gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   GatA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRATIO1112

   Plasmid

   #141066

   Purpose

   Gateway cloning compatible dual fluorescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Use

   Tags

   mScarlet-I and mVenus

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIntegrase_10

   Plasmid

   #60581

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSY14-ptb

   Plasmid

   #90490

   Purpose

   pSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

   Depositor

   Sheng Yang

   Article

   Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

   Insert

   sceC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL690

   Plasmid

   #47753

   Purpose

   Expresses dCas9-VP64 fusion

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   CMV

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

   Plasmid

   #84249

   Purpose

   Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgSV40
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG25-L-IFP-F[1] (clon 3)

    Plasmid

    #52899

    Purpose

    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[1]
    

    Use

    Vector for homologous recombination

    Tags

    Expression

    Mutation

    Promoter

    TEF promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pAG32-L-IFP-F[2] (clon 3)

    Plasmid

    #52900

    Purpose

    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[2]
    

    Use

    Vector for homologous recombination

    Tags

    Expression

    Mutation

    Promoter

    TEF promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLenti_9xD3B_Cluc_Norm

    Plasmid

    #68414

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBPHLWL-GAL4DBD

    Plasmid

    #26256

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    ZIP- Gal4DBD (GAL4 Budding Yeast)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRATIO2212

    Plasmid

    #141302

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    mScarlet-I and mVenus

    Expression

    Plant

    Mutation

    Promoter

    UBQ10 promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pReporter_2

    Plasmid

    #60563

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Clover-Lifeact-7

    Plasmid

    #54551

    Purpose

    Localization: Actin, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. Kohinoor/pcDNA3

    Plasmid

    #67771

    Purpose

    Kohinoor for mammalian cell expression in cytosol

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. PH-dL5**

    Plasmid

    #96946

    Purpose

    PLC pleckstrin homology domain linked to FAP for CALI validation

    Depositor

    Marcel Bruchez

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    Pleckstrin homology domain
    

    Use

    Tags

    dL5 and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-PH-dL5**

    Plasmid

    #96947

    Purpose

    Expresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validation

    Depositor

    Marcel Bruchez

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    Pleckstrin homology domain
    

    Use

    Tags

    EGFP and dL5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-6NBC-GFP-PGK-Puro-Barcode1

    Plasmid

    #83678

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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