Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 15,944 results for: POR

Showing: 2481 - 2500 of 15944 results

  2. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid
    #117699
    Purpose
    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
    Depositor
    Joerg Mansfeld
    Article
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    Use
    Tags
    HA tag, Myc-NES, and NLS
    Expression
    Mammalian
    Mutation
    codon optimized for murine expression
    Promoter
    None, 2A peptide from porcine teschovirus-1 polyp…
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    117699-plasmid-map-sequence-id-368109

    Enlarge

  3. pKrox24(MapErk)Luc

    Plasmid
    #200112
    Purpose
    Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    MapErk sequence in minimal promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    highly modified sequence
    Promoter
    5 copies of designed MapErk sequence in minimal p…
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    200112-plasmid-map-sequence-id-399196

    Enlarge

  5. pSPObooster

    Plasmid
    #216160
    Purpose
    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains
    Depositor
    Grant Brown
    Article
    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978
    Inserts
    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    MKT1 gene containing an aspartic acid to glycine …
    Promoter
    endogenous promoter
    Available since
    May 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216160-plasmid-map-sequence-id-433867

    Enlarge

  6. Rlucrarr3-Flash5

    Plasmid
    #74133
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    cyt-GlycoID-V5
    Use
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184640-plasmid-map-sequence-id-366751

    Enlarge

  11. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174644
    Purpose
    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Venus-SSPB and iLID-mCherry
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174644-plasmid-map-sequence-id-347042

    Enlarge

  15. pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

    Plasmid
    #217676
    Purpose
    AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.
    Depositor
    Joseph Raimondo
    Article
    Selfe et al bioRxiv 2023.08.30.555464
    Insert
    GtACR2-P2A-Voltron2ST
    Use
    AAV
    Tags
    Soma targeting sequence (Kv2.1) on Voltron2 gene …
    Expression
    Mammalian
    Mutation
    Promoter
    EF‐1α
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217676-plasmid-map-sequence-id-432468

    Enlarge

Showing: 2481 - 2500 of 15944 results