We narrowed to 4,390 results for: gca
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Plasmid#171214PurposeGateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.DepositorInsertBNT162b2ΔUTR
UseGateway cloningMutationKozak sequence changed from CCCGCCACC to GGCTGCAC…Available SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL049
Plasmid#198809PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.DepositorInsert15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
ExpressionWormAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6.shRNA-Sdc1-1376
Plasmid#195574PurposeExpresses shRNA to knockdown mouse Syndecan-1DepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1-p2A-eGFP
Plasmid#158699PurposeMammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection markerDepositorInsertscFLARE1-p2A-eGFP
UseAAVTagsFlag, V5. eGFPExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS1
Plasmid#196980PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS1 tags
ExpressionPlantPromoterpU6-26, pU6-29Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP
Plasmid#198863PurposePlasmid encoding for 2 gRNAs targeting the human TERT geneDepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-pX330-Cas9-Blast
Plasmid#159741PurposeExpresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.DepositorInsertdonor plasmid targeting sgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1#32-Tet-pLKO-puro
Plasmid#107399PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc17a7
Plasmid#124846PurposeMutagenesis of Slc17a7DepositorInsertSlc17a7 (Slc17a7 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 shCAV1-NLS-mCherry
Plasmid#187257PurposeLentiviral expression of shRNA targeting Caveolin1 and NLS-mCherryDepositorAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-33-MC_hammer
Plasmid#138990PurposeIVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-32-riot_punisher
Plasmid#138988PurposeIVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-32-riot_punisher
Plasmid#138987PurposeIVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-31-murderous_crow
Plasmid#138986PurposeIVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS1
Plasmid#140624PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 U6-SaAi9L-U6-SaAi9R
Plasmid#78604PurposeAAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassetteDepositorInsertgRNAs for SaCas9 targeting Ai9 locus
UseAAV and CRISPRExpressionMammalianPromoterU6Available SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Actb KI #2
Plasmid#139666PurposeEndogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)DepositorInsertgRNA and GFP donor
ExpressionMammalianPromoterU6 and CbhAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_mic
Plasmid#184916PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE unc13a GFP KI
Plasmid#131498PurposeEndogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)DepositorAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Kan-ADE2
Plasmid#232105PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only