We narrowed to 16,209 results for: She

1. pUS252s

   Plasmid

   #191833

   Purpose

   Expresses fuGFPs constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPs to place in other plasmid backbones

   Depositor

   Nicholas Coleman

   Insert

   fuGFPs
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSV40-GUS-P2A-Hygro

   Plasmid

   #106480

   Purpose

   Expression of GUS and Hygromycin resistance gene from SV40 promoter

   Depositor

   Alex Hewitt

   Inserts

   beta-glucuronidase
   Hygromycin B resistance
   

   Use

   Tags

   Expression

   Mammalian and Plant

   Mutation

   Promoter

   SV40 promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pxCas9CR4

   Plasmid

   #111656

   Purpose

   PCas9CR4 with the xCas9 mutations allowing NG PAM sites

   Depositor

   Christopher Reisch

   Insert

   TetR and xCas9
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   xCas9 mutations

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLENTI_TetOn_Hu_MAD2(WT)

   Plasmid

   #136347

   Purpose

   Lentiviral inducible expression (TetOn) of human MAD2

   Depositor

   Guillermo de Cárcer

   Insert

   MAD2L1 (MAD2L1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-TRC.mKO2_shHuR CDS

   Plasmid

   #110426

   Purpose

   TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAVL1 HuR
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (RNA Pol III)

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. KDM4DL

   Plasmid

   #38990

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KDM4DL (KDM4E Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. Pf-GMPS

   Plasmid

   #36899

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Pf-GMPS (PF10_0123 Plasmodium falciparum)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

8. PX396 Parvibaculum lavamentivorans Cas9

   Plasmid

   #68333

   Purpose

   Parvibaculum lavamentivorans Cas9

   Depositor

   Feng Zhang

   Insert

   PlCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. FFTTH--GFP-Amotl2

   Plasmid

   #112833

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Insert

   Amotl2 (Amotl2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PIM1

    Plasmid

    #39132

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PIM1 (PIM1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pTCF79 H-2D(b) (CD8 mut)

    Plasmid

    #180453

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    H-2D(b) (CD8 mut)
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    D227K, replacement of transmembrane domain with e…

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pExp-DsbA

    Plasmid

    #112562

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-DsbA(AA20-208) and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-LacZ-BbsI

    Plasmid

    #114671

    Purpose

    For one-step cloning (with blue/ white screening) of an open reading frame of interest for constitutive gene expression

    Depositor

    Lynne Postovit

    Insert

    LacZ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Lac promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL1423

    Plasmid

    #194904

    Purpose

    pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparum

    Depositor

    Scott Lindner

    Insert

    TurboID
    

    Use

    Plasmodium falciparum

    Tags

    GFPmut2

    Expression

    Mutation

    Promoter

    None. This allows a C-terminal fusion to a protei…

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX377 Corynebacter diphtheriae Cas9

    Plasmid

    #68314

    Purpose

    Corynebacter diphtheriae Cas9

    Depositor

    Feng Zhang

    Insert

    CdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG497 mito-iFAST

    Plasmid

    #130819

    Purpose

    Expresses mito-iFAST in mammalian cells (mitochondria labeling)

    Depositor

    Arnaud Gautier

    Insert

    mito-iFAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. TAP-GFP-Amotl2

    Plasmid

    #112835

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Insert

    Amotl2 (Amotl2 Mouse)
    

    Use

    Tags

    GFP and TAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)

    Plasmid

    #48292

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX405 Neisseria meningitidis Cas9

    Plasmid

    #68702

    Purpose

    Neisseria meningitidis Cas9

    Depositor

    Feng Zhang

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pENTR_Hu MAD2(WT)

    Plasmid

    #136343

    Purpose

    Human MAD2 cDNA in Gateway pENTR

    Depositor

    Guillermo de Cárcer

    Insert

    MAD2L1 (MAD2L1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET His6 MBP TEV expression vector with BioBrick polypromoer restriction sites (14-C)

    Plasmid

    #48309

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pXP420 v2 (repaired Amp promoter)

    Plasmid

    #86920

    Purpose

    Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.

    Depositor

    Gary Yellen

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDS-Red-N1 GABAA beta3

    Plasmid

    #109143

    Purpose

    The expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.

    Depositor

    Stefano Vicini

    Insert

    GABAa receptor
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    beta3 subunit of the GABAa receptor

    Promoter

    CMV

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCB52

    Plasmid

    #69747

    Purpose

    Retroviral expression of Cyclin B1

    Depositor

    Iain Cheeseman

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

25. PX394 Neisseria cinerea Cas9

    Plasmid

    #68331

    Purpose

    Neisseria cinerea Cas9

    Depositor

    Feng Zhang

    Insert

    NcCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MUT

    Plasmid

    #38872

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MUT (Mut Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pBS L30 mRuby3-Rab11A

    Plasmid

    #166430

    Purpose

    Rab11A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab11A
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. STK10

    Plasmid

    #38830

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    STK10 (stk10 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

29. dCas9-3×RFP-P2A-CASANOVA/gRNA telo

    Plasmid

    #137711

    Purpose

    Vector co-expressing dCas9-3×RFP-P2A-CASANOVA and sgRNA telo

    Depositor

    Dominik Niopek

    Insert

    dCas9-3×RFP-P2A-CASANOVA/gRNA telo
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. cp193g7N

    Plasmid

    #25982

    Depositor

    Robert Campbell

    Article

    Carlson et al Protein Sci. 2010 Jun 2. ():.

    Insert

    cp193g7N
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

31. pRK GFP Paxillin Y31A

    Plasmid

    #50530

    Depositor

    Kenneth Yamada

    Insert

    Paxillin (PXN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y31A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pKM510

    Plasmid

    #134227

    Purpose

    Retroviral plasmid expressing mCherry - HJURP

    Depositor

    Iain Cheeseman

    Insert

    mCherry-HJURP-LacI 1-304 (HJURP Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAc5.1B-EGFP-DmDis3

    Plasmid

    #66592

    Purpose

    expresses EGFP-tagged Dis3 in Drosophila cells

    Depositor

    Elisa Izaurralde

    Insert

    DIS3 (Dis3 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Actin 5C

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo

    Plasmid

    #137712

    Purpose

    Vector co-expressing dCas9-3×RFP-P2A-wtAcrIIA4 and sgRNA telo

    Depositor

    Dominik Niopek

    Insert

    dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAGGS-S-B.1.1.7-FLAG

    Plasmid

    #177093

    Purpose

    B.1.1.7 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein B.1.1.7 (S SARS-CoV-2)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid

    #195487

    Purpose

    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    HaloTag.v7
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C61T, C262V

    Promoter

    T7

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PX406 Pasteurella multocida Cas9

    Plasmid

    #68703

    Purpose

    Pasteurella multocida Cas9

    Depositor

    Feng Zhang

    Insert

    PmCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C)

    Plasmid

    #48286

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mT2A-td-sfGFP-FNF

    Plasmid

    #182339

    Purpose

    Recombineering cassette plasmid for T2A-td-sfGFP-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-td-sfGFP-FNF
    

    Use

    Mouse Targeting; Flp / frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge