We narrowed to 5,312 results for: cycs

1. pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

   Plasmid

   #113695

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-Puro-FH-AGO2-S824E-S828E

   Plasmid

   #91999

   Purpose

   Expresses FLAG-HA-AGO2 (S824E-S828E)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S828E

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-Puro-FH-AGO2-S824E-S831E

   Plasmid

   #92000

   Purpose

   Expresses FLAG-HA-AGO2 (S824E-S831E)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S831E

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Puro-FH-AGO2-S828E-S831E

   Plasmid

   #92001

   Purpose

   Expresses FLAG-HA-AGO2 (S828E-S831E)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S828E, S831E

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-Puro-FH-AGO2-S824E-S828E-S831E

   Plasmid

   #92002

   Purpose

   Expresses FLAG-HA-AGO2 (S824E-S828E-S831E)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S828E, S831E

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-Puro-FH-AGO2-S824A-T830A

   Plasmid

   #92003

   Purpose

   Expresses FLAG-HA-AGO2 (S824A-T830A)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824A, T830A

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPC-S22AProgerin-CS

   Plasmid

   #69063

   Purpose

   encodes a Progerin mutant with serine 22 mutated to an alanine and with a substitution of cysteine in CaaX-motif by a serine

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

   Insert

   Progerin (LMNA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Progerin with serine 22 mutated to alanine and wi…

   Promoter

   CMV

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. FLAG-SKP2

   Plasmid

   #215092

   Purpose

   Expresses FLAG-SKP2 in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   SKP2 (SKP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-MCS(extended)-Puro (empty vector)

   Plasmid

   #172603

   Purpose

   Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The multiple cloning site (MCS) has been edited/e…

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

    Plasmid

    #223965

    Purpose

    Dual fluorescent reporter for histone H2B and for Cdt1

    Depositor

    Hee Won Yang

    Article

    Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

    Insert

    H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670 and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-AMBRA1-3xFLAG

    Plasmid

    #172605

    Purpose

    Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

    Plasmid

    #113691

    Purpose

    U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-ERalpha

    Plasmid

    #215093

    Purpose

    Expresses HA-ERalpha in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-POLE1

    Plasmid

    #215140

    Purpose

    Expresses mCherry-POLE1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    POLE1 (POLE Human)
    

    Use

    Retroviral

    Tags

    mCherry_FWR

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acid 80-86 and 211-217 …

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

    Plasmid

    #65458

    Purpose

    Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-APEX2

    Plasmid

    #215127

    Purpose

    Expresses mCherry-APEX2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    APEX2 (APEX2 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. tetO-HAND2

    Plasmid

    #170690

    Purpose

    doxycycline-inducible overexpression of HAND2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    HAND2 (HAND2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EMTB-TurboID-V5-puro

    Plasmid

    #190741

    Purpose

    A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    EMTB-TurboID-V5 (RPS14 EMTB is human ensconsin; TurboID is engineered BirA from E.coli, Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1 blast OFF 3xflag IREB2

    Plasmid

    #169889

    Purpose

    Express 3x flag IREB2, suppressible by doxycycline addition

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    IREB2 (IREB2 Human)
    

    Use

    Lentiviral; Doxycycline mediated suppression

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Silent mutations in sgIREB2_1 and sgIREB2_2 targe…

    Promoter

    TRE

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-CDT2

    Plasmid

    #215091

    Purpose

    Expresses FLAG-CDT2 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDT2 (DTL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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