We narrowed to 31,661 results for: uros

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pDNR-mCherry-T2A-3xPuroR-Frame 0

Plasmid

#206991

Purpose

Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_attP-Ef1a-Puro-P2A-mCherry

Plasmid

#247159

Purpose

attP donor plasmid expressing puromycin resistance and mCherry

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

Plasmid

#223160

Purpose

Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

dGFP-P2A-NanoLuc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 0819

Plasmid

#118704

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 2 tet pLKO puro

Plasmid

#162984

Purpose

Tet-inducible shRNA targeting human METTL3 #2

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VP64-Puro

Plasmid

#99371

Purpose

3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-VP64-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMK231 (AAVS1 CMV-MCS-PURO)

Plasmid

#105924

Purpose

Donor for integration at the human AAVS1 locus

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-SV40-puro-EF1a-HA-NLGN1-bc

Plasmid

#200167

Purpose

Expresses HA-tagged human NLGN1

Depositor

Paul Blainey

Article

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Insert

Neuroligin-1 (NLGN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

Plasmid

#232880

Purpose

Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

Depositor

Paul DeCaen

Article

DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

Insert

ADP-ribosylation factor-like protein 13B (ARL13B Human)

Use

Lentiviral

Tags

GFP and RGECO1

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG CLTX-CAR

Plasmid

#157743

Purpose

knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

chlorotoxin containing chimeric antigen receptor targeting glioblastoma

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO eGFP Puro (w159-1)

Plasmid

#17481

Purpose

3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHDR-FOXL2-T2A-tdTomato-PuroTK

Plasmid

#192892

Purpose

HDR donor for inserting FOXL2-T2A-tdTomato reporter

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2-tdTomato (FOXL2 Human)

Use

CRISPR

Mutation

T2A-tdTomato fluorescent reporter

Promoter

PGK

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-Puro_hCOL4A2-Myc-His-WPRE

Plasmid

#198080

Purpose

Express hCOL4A2 in mammalian cells

Depositor

Jeffrey Miner

Insert

COL4A2 (COL4A2 Human)

Tags

TEV-Myc-His

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

Plasmid

#115519

Purpose

To construct multiple sgRNA expression vector for CRISPR

Depositor

Xingxu Huang

Insert

ccdB-sgRNA scaffold

Use

CRISPR

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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iF58 PB-Zim3-dCas9-mScarlet-Puro

Plasmid

#204728

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Ef1a-hMGT-PGK-puro

Plasmid

#172394

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-EEA-5guides-PGK-Puro

Plasmid

#102898

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-5guides-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-EcSTH-FLAG

Plasmid

#218628

Purpose

This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

EcSTH

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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