We narrowed to 2,596 results for: ubiquitin

1. XE257 pCDNA3.1(zeo)-hDsh3-truncDEP

   Plasmid

   #16752

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3-trunc before DEP (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncated before DEP

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6p1 AO7RE

   Plasmid

   #137843

   Purpose

   GST tagged AO7 encoding aa 126-258 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT-ubb-cytoBirA-2a-mCherry

   Plasmid

   #79885

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5FRT/TO-Strep/HA-UBE2QL1

   Plasmid

   #124665

   Purpose

   Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Use

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET151-GST-PP-RPN13 (19-132)

   Plasmid

   #73742

   Purpose

   Expression of human RPN13 PRU domain in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   PRU domain only (residues 19-132)

   Promoter

   T7

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pGEXNKI-GST-3C-LIC

   Plasmid

   #108710

   Purpose

   Expression of a GST-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   GST-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-Neo-gp78 R2M / JM21

   Plasmid

   #13304

   Depositor

   Allan Weissman

   Article

   Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

   Insert

   gp78 (AMFR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C356G ; H362A

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pGEX6P-1 human HUWE1 WWE (1611-1703)

   Plasmid

   #132614

   Purpose

   expresses human HUWE1 WWE (1611-1703) in E. coli

   Depositor

   Wenqing Xu

   Article

   Wang et al Genes Dev. 2012 Feb 1;26(3):235-40. doi:

   Insert

   HUWE1 WWE (1611-1703)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-His6-Ubc9-C93A

   Plasmid

   #133910

   Purpose

   Bacterial expression of His6-Ubc9-C93A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 93 to Alanine

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5FRT/TO-Strep/HA-UBE2Q2

    Plasmid

    #124668

    Purpose

    Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2Q2 (UBE2Q2 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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