We narrowed to 29,541 results for: REP

1. mKOkappa-2A-mTurquoise2

   Plasmid

   #98837

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mKOkappa-2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKO (Hyg)

   Plasmid

   #110087

   Purpose

   Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-T2A-moxMaple3

   Plasmid

   #120875

   Purpose

   Mammalian expression of mCherry and moxMaple3

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

   Inserts

   mCherry
   moxMaple3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   from mMaple3, moxMaple3 was engineered by replaci…

   Promoter

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-FRT-GFP-mCherry-3pGW

   Plasmid

   #53965

   Purpose

   Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

   Inserts

   GFP
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.3 EGFP

   Plasmid

   #115786

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRLSIN.cPPT.PGK.NeuroD2.WPRE

   Plasmid

   #122054

   Purpose

   Lentiviral expression of NeuroD2

   Depositor

   Stephen Tapscott

   Article

   Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

   Insert

   NeuroD2 (Neurod2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSETA-qmTQ2-citrate

   Plasmid

   #225251

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-citrate
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMK449 (BromoTag-mClover-BSD)

   Plasmid

   #214379

   Purpose

   BromoTag-mClover for C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BromoTag-mClover-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-Gdf2

   Plasmid

   #163595

   Purpose

   Expresses GDF-2 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Gdf2 (Gdf2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-mWasabi-G

    Plasmid

    #164161

    Purpose

    Expresses mWasabi under the control of CAG promoter, basic vector, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-NLS-ZapCV2-ALA

    Plasmid

    #203665

    Purpose

    Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2-Ala zinc dead sensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Cys and His in Zap1 is mutated with Ala

    Promoter

    CMV promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101104

    Purpose

    Encodes dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

    Plasmid

    #188703

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET23a-HsRPS27a

    Plasmid

    #69561

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pMK448 (BromoTag-BSD)

    Plasmid

    #214378

    Purpose

    BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. eGFP L138 gRNA

    Plasmid

    #119133

    Purpose

    gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    eGFP L138 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    U6

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LeGO-iG2-wPRE-pA

    Plasmid

    #60489

    Purpose

    eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)

    Depositor

    Boris Fehse

    Article

    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

    Insert

    IRES-eGFP; wPRE; BGH-pA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC57-PP7v3

    Plasmid

    #107230

    Purpose

    Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    24xPP7v3
    

    Use

    Tags

    3x FLAG-tag and P2A site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HI.fate.SFFV-E

    Plasmid

    #191555

    Purpose

    Monitor HIV-1 fate

    Depositor

    Joseph Sodroski, Alon Herschhorn

    Article

    Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

    Insert

    HIV-1 incomplete genome
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    env-deleted + vpr mutation(s)

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HI.fate.E

    Plasmid

    #191553

    Purpose

    Monitor HIV-1 fate

    Depositor

    Joseph Sodroski, Alon Herschhorn

    Article

    Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

    Insert

    HIV-1 incomplete genome
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    env-deleted + vpr mutation(s)

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge