We narrowed to 34,434 results for: CaS;

1. pcDNA3.1 iCasper T2A HO1

   Plasmid

   #64278

   Purpose

   Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

   Depositor

   Xiaokun Shu

   Article

   To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-dCas13-inactive M3M14nes

   Plasmid

   #157855

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-dCasRx-ALKBH5-PURO

   Plasmid

   #175582

   Purpose

   Expresses dCasRx-ALKBH5 in mammalian cell

   Depositor

   Qi Xie

   Article

   Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

   Insert

   dCasRx-ALKBH5
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   RfxCas13d R239A/H244A/R858A/H863A

   Promoter

   pMSCV

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-dCasRx-METTL3-PURO

   Plasmid

   #174218

   Purpose

   Expresses dCasRx-METTL3 in mammalian cell

   Depositor

   Qi Xie

   Article

   Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

   Insert

   dCasRx-METTL3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMSCV

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dual-CRISPR-Cas9-U6-H1 (pBP48)

   Plasmid

   #218932

   Purpose

   The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   2A-Puro and 3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SpCas9-P2A-puro

   Plasmid

   #214358

   Purpose

   Expresses SpCas9 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9 and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

   Plasmid

   #196197

   Purpose

   Cas9 + gRNA expression to edit G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-TurboCas-NLS-Blast

   Plasmid

   #232346

   Purpose

   Lentiviral expression vector for dCas9 fused to miniTurboNLS

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA, miniTurbo, V5 and 3xSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28b-Cas9-His

    Plasmid

    #47327

    Purpose

    For in vitro expression and purification of Cas9 protein

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-dCas9-KRAB-blast

    Plasmid

    #89567

    Purpose

    Plasmid expression dCas9 protein in fusion with KRAB domain

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    Blasticidin resistance gene and KRAB

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TRE-dCas9-KRAB-MeCP2

    Plasmid

    #122267

    Purpose

    SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    dCas9-KRAB-MeCP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0041-RanCas13b crRNA backbone

    Plasmid

    #103852

    Purpose

    For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-RanCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. HiFi-nCas9-NG_P2A-mCherry

    Plasmid

    #219936

    Purpose

    Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    HiFi-nCas9(D10A/R691A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX303-ZIM3-KRAB-dCas9

    Plasmid

    #154472

    Purpose

    Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC013 - Twinstrep-SUMO-huLwCas13a

    Plasmid

    #90097

    Purpose

    Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a
    

    Use

    CRISPR

    Tags

    6xHis-Twin Strep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-enCas12a-ABE8e-V106W

    Plasmid

    #193653

    Purpose

    Expression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-TadA*8e-V106W, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, TadA*8e-V106W

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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