We narrowed to 10,978 results for: Vars;

1. pET-3a-His-MBP-TEV-VD_Cas1_WT

   Plasmid

   #120365

   Purpose

   Expresses wildtype V-D Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VD_Cas1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pVC-CRISPR1

   Plasmid

   #120366

   Purpose

   Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   CRISPR1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVC-CRISPR

   Plasmid

   #120369

   Purpose

   Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Inserts

   CRISPR1
   CRISPR2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVD_CRISPR

   Plasmid

   #120370

   Purpose

   Plasmid containing shortened version of V-D CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VD_CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLK25

   Plasmid

   #37255

   Depositor

   Ann Hubbard

   Article

   Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

   Insert

   Scribble (SCRIB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 727-1630

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pUAS-MmTFP13xmyc

   Plasmid

   #86371

   Purpose

   membrane targeted Tfp1 expression in zebrafish

   Depositor

   Rachel Wong

   Article

   Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

   Insert

   mTfp1
   

   Use

   Zebrafish

   Tags

   3xMyc and GAP43 membrane targeting

   Expression

   Mutation

   Promoter

   UAS

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SNAP-EF(N)-5nmSAH-GFP(1-10)

   Plasmid

   #61659

   Purpose

   5nm SAH scaffold with EF Hand and split GFP attachment sites

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   SNAP-EF(N)-5nmSAH-GFP(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLK67

   Plasmid

   #37251

   Depositor

   Ann Hubbard

   Article

   Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

   Insert

   Scribble (SCRIB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-728

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pSopBWT

   Plasmid

   #112512

   Purpose

   PsopB driven sopB and sigE

   Depositor

   Olivia Steele-Mortimer

   Article

   Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

   Insert

   sopB/sigE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PsopB

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQP-NiRISB

    Plasmid

    #138977

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCR-U6-gRNA-miniCMV-TdTomato

    Plasmid

    #192656

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-oChEF-citrine

    Plasmid

    #50975

    Purpose

    AAV transfer vector with mammalian codon-optimized ChEF-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    ChEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mEGFP-30nmSAH-GFP(1-10)

    Plasmid

    #61677

    Purpose

    GFP-labeled SAH scaffold with split GFP attachment site

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    mEGFP-30nmSAH-GFP(1-10)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57-8xHis

    Plasmid

    #101699

    Purpose

    8xHis in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    AATG-[8xHis]-AGCC
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLK27

    Plasmid

    #37256

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    Scribble (SCRIB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1197-1630

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-6P1-OPNa-delta S

    Plasmid

    #107983

    Purpose

    bacterial expression of full-length OPN

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    OPNa 1080bp-17aa signal (SPP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAVS1-TRE3G-NODAL-matMYC

    Plasmid

    #115639

    Purpose

    For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTagRFP675-actin-C1

    Plasmid

    #44277

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    CMV

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330A_FokI-1x4

    Plasmid

    #63604

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTEF-Sup35C

    Plasmid

    #1202

    Depositor

    Susan Lindquist

    Article

    True et al Nature 2004 Sep 9;431(7005):184-7. Epub

    Insert

    Sup35 C-terminus (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Sup35 C-terminus ~254aa-end

    Promoter

    TEF

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge