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pWN_U6-TRAC-miniGag

Plasmid

#228960

Purpose

miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4

Plasmid

#58316

Purpose

FLAG-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pLX-V5-TRAF2-puro

Plasmid

#44111

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral

Tags

V5

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pTRANS_232

Plasmid

#91121

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_102

Plasmid

#91200

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_150

Plasmid

#91205

Purpose

Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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EmptyTranscriptionalReporter

Plasmid

#62453

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pWN_U6-TRAC-miniGag-Cas9

Plasmid

#228959

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-TRAF6-C70A

Plasmid

#43926

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

TNF Receptor-Associated Factor 6 (Traf6 Mouse)

Tags

2x FLAG

Expression

Mammalian

Mutation

C70A

Available Since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-myc-His wt-Trask

Plasmid

#31768

Depositor

Mark Moasser

Article

Spassov et al PLoS One. 2011 Apr 29;6(4):e19154.

Insert

Trask (CDCP1 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pUltra-H2B-mTagBFP2

Plasmid

#161791

Purpose

3rd generation lentiviral multicistronic vector for mammalian expression of EGFP, H2B-mTagBFP2, and one gene of interest

Depositor

Jorge Luis Arias-Arias

Insert

H2B-mTagBFP2

Use

Lentiviral; Multicistronic

Tags

EGFP and mTagBFP2

Expression

Mammalian

Mutation

I174A in the mTagBFP2 protein according to PLoS O…

Promoter

UbC

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-TRAF6-C70A

Plasmid

#43930

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

TNF Receptor-Associated Factor 6 (Traf6 )

Tags

GST

Expression

Bacterial

Mutation

C70A

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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Lactotransferrin-CD4d3+4-bio

Plasmid

#73124

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Lactotransferrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Lactotransferrin (LTF Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAdTrack-CMV HA ALAS-1

Plasmid

#22746

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

ALAS-1 (Alas1 Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MYO10-HMM-Nanotrap

Plasmid

#87255

Purpose

Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Tags

GFP-nanotrap

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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1200 pAdTrack FKHR H215R

Plasmid

#9038

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR H215R (FOXO1 Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

H215R

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pSF11-wElectra1

Plasmid

#184932

Purpose

Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1

Expression

Worm

Promoter

tag-168

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pActin-Electra2

Plasmid

#184942

Purpose

beta-actin labelling with blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2-beta-actin

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pUltra-FPheKRS

Plasmid

#136631

Purpose

Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteins

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

FPheKRS

Expression

Bacterial

Promoter

Tac1 promoter

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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sgTrack-Gateway

Plasmid

#114011

Purpose

3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNA

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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