We narrowed to 31,550 results for: Eng;

1. pML107-PMR1

   Plasmid

   #226264

   Purpose

   Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   PMR1 gRNA (PMR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS316-SSD1-NCYC110

   Plasmid

   #226279

   Purpose

   Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   SSD1 (SSD1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDLterm

   Plasmid

   #211903

   Purpose

   Base plasmid for dual-luciferase constructs to measure terminator strength

   Depositor

   Stan Fields

   Article

   Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S enhancer + 35S minimal promoter

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Zhou-pLOV-GFP-Luc

   Plasmid

   #161030

   Purpose

   Lentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection.

   Depositor

   Dapeng Zhou

   Article

   Tong et al Vaccines (Basel). 2021 Mar 22;9(3). pii:

   Insert

   GFP and luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJH4693

   Plasmid

   #200784

   Purpose

   Ptwk-40s EGFP unc-54 3' UTR C.elegans AVA and other neurons expression of EGFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   no
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Ptwk-40s

   Available since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pJH4506

   Plasmid

   #200255

   Purpose

   Pnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   TeTx
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   Promoter

   Pnpr-4

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH2673

   Plasmid

   #199691

   Purpose

   Pnmr-1 FLPe unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of FLPe

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   flpE (flpE )
   

   Use

   Tags

   no

   Expression

   Worm

   Mutation

   Promoter

   Pnmr-1 long

   Available since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. TtA_pET29b

   Plasmid

   #215432

   Purpose

   Expression construct for TtA gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   TtA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDonor_RB-TnV2_pJEx

   Plasmid

   #213908

   Purpose

   Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGAL1-VP1 (LEU2)

    Plasmid

    #182480

    Purpose

    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLS.544

    Plasmid

    #184961

    Purpose

    Test effect of a1/a2 length on editing murF using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    murF donor G1359A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ND6-L1397-N_UGI-NES_right

    Plasmid

    #187846

    Purpose

    expresses the right-side half ND6-UGI-NES in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ND6-L1397-N_UGI-NES_left

    Plasmid

    #187845

    Purpose

    expresses the left-side half ND6-UGI-NES in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNCS-Clover4-LUMABS-HA

    Plasmid

    #183486

    Purpose

    To express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody detection.

    Depositor

    Jun Chu, Zongwen Jin

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-HA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ-NF1-L1-dSpRY

    Plasmid

    #177681

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ-NF1-L2-dSpRY

    Plasmid

    #177682

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ-NF-L2-dMb2Cas12a

    Plasmid

    #177684

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible foki-dmb2cas12a entry …

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Pb mD3S4-dN

    Plasmid

    #180252

    Purpose

    P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

    Depositor

    Timothy Springer

    Article

    Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

    Insert

    D3 of P. berghei HAP2 with N-glycosylation sites mutated
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    N516T, S533N and N539Q

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T-1-Rnt1-dsRBD(+6A)

    Plasmid

    #171552

    Purpose

    Encoding part of the budding yeast Rnt1 protein from L366 to S453

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Rnt1 (RNT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    a six-alanine insertion in the L1 region

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-4T-1-Rnt1-dsRBD(+3A)

    Plasmid

    #171551

    Purpose

    Encoding part of the budding yeast Rnt1 protein from L366 to S453

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Rnt1 (RNT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    a three-alanine insertion in the L1 region

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX-4T-1-Rnt1-dsRBD(+6)

    Plasmid

    #171550

    Purpose

    Encoding part of the budding yeast Rnt1 protein from L366 to S453

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Rnt1 (RNT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    a six amino acid_TRLTEG_insertion in the L1 region

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-4T-1-Rnt1-dsRBD

    Plasmid

    #171549

    Purpose

    Encoding part of the budding yeast Rnt1 protein from L366 to S453

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Rnt1 (RNT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX-4T-1-Xlrbpa

    Plasmid

    #171547

    Purpose

    Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Xlrbpa (prkra.L Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pre_II_CS3_7746_v3

    Plasmid

    #126856

    Purpose

    7653 b scaffold from compatibility group 3, made by method II, containing 2 Zn-dependent self-cleaving DNAzyme cassette for lbisection into 2 3780 b scaffolds

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    II_CS3_7746_v3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHIs8-4b-SaDAH::G226D

    Plasmid

    #140130

    Purpose

    Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli

    Depositor

    Jing-Ke Weng

    Article

    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

    Insert

    Dechloroacutumine halogenase G226D variant from Sinomenium acutum
    

    Use

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    changed Gly226 to Asp226

    Promoter

    T7

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tol2-lyzC-miR-100-1-dendra2

    Plasmid

    #105438

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    miR-100-1 Dendra2 (mir100-1 Zebrafish)
    

    Use

    Zebrafish

    Tags

    N/A

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFB HTB_hMSH6_T1219D

    Plasmid

    #135466

    Purpose

    Expression human MSH6_T1219D mutant protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6_T1219D (MSH6 Human)
    

    Use

    Tags

    His-tag

    Expression

    Insect

    Mutation

    changed threonine to aspartic acid

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTK-EdnrbE2-Cerulean

    Plasmid

    #127771

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE2
    

    Use

    Enhancer reporter construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTK-EdnrbE1-Citrine

    Plasmid

    #127773

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE1
    

    Use

    Enhancer reporter construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pU6-Sox3gRNA-T2

    Plasmid

    #127778

    Purpose

    Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    sox3gRNAT2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLY133

    Plasmid

    #130961

    Purpose

    Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTK_Cerulean_Nanotag_92_positive_Control

    Plasmid

    #130573

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    FoxD3_cranial_neural_crest_enhancer_NC4
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTK_Cerulean_Nanotag_91_positive_Control

    Plasmid

    #130572

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    FoxD3_cranial_neural_crest_enhancer_NC3
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLY86

    Plasmid

    #130949

    Purpose

    Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA2-WTmis2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plux2

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLY132

    Plasmid

    #130960

    Purpose

    Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLY130

    Plasmid

    #130952

    Purpose

    Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. hsa-miR 19b plix 4.03

    Plasmid

    #121126

    Purpose

    inducible expression in cells

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    hsa-miR-19b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. hsa-miR199 plix 4.03

    Plasmid

    #121129

    Purpose

    inducible expression in cells

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    hsa-miR-199
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hsa-miR-199-dendra2 PCDNA

    Plasmid

    #121133

    Purpose

    mammalian cell expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    hsa-miR-199
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Tol2-lyzC-microRNA 301a-Dendra2

    Plasmid

    #97126

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    microRNA 301a Dendra2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge