We narrowed to 7,762 results for: loxP

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EF1a_DIO_mMeCP2-HaloTag

Plasmid

#164062

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoter

Depositor

Nathaniel Heintz

Publication

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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Per2:iLuc

Plasmid

#160293

Purpose

target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.

Depositor

Joseph Takahashi

Publication

Shan et al Neuron. 2020 Aug 4. pii: S0896-6273(20)3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Per2LUC

Expression

Mammalian

Promoter

no promotor

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS-PE2-attB

Plasmid

#149550

Purpose

Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

Depositor

Norbert Perrimon

Publication

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Expression

Insect

Promoter

UAS

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF17_P2A_Hygro_Barcode

Plasmid

#120503

Purpose

Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF17 (KLF17 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MITF_P2A_Hygro_Barcode

Plasmid

#120460

Purpose

Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MITF (MITF Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_LMX1A_P2A_Hygro_Barcode

Plasmid

#120457

Purpose

Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LMX1A (LMX1A Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_LHX3_P2A_Hygro_Barcode

Plasmid

#120456

Purpose

Barcoded lentiviral vector to express LHX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LHX3 (LHX3 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCXLE-dCas9VPH-T2A-GFP-shP53

Plasmid

#102895

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Publication

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9VPH-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [Chronos-tdTomato]

Plasmid

#84481

Purpose

AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

human synapsin promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQFKan

Plasmid

#32321

Depositor

Steve Stowers

Publication

Stowers et al Fly (Austin). 2011 Oct 1;5(4).

Insert

QF Kan

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_NUP214:1-2090

Plasmid

#211294

Purpose

Mammalian expression of full-length human NUP214. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NUP214:M1-S2090 (NUP214 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

P574S natural variant (VAR_045691). S1872N annot…

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ZnF-hSyn-DIO-GPHNFingR-eGFP

Plasmid

#200279

Purpose

Genetically encoded fluorescent probe for labelling endogenous inhibitory synapses in vivo

Depositor

Peter Scheiffele

Publication

Okur et al bioRxiv 2023

Insert

zFn-FingR-GPHN-eGFP

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

human Synapsin

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -2

Plasmid

#180009

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-LexGAD-3xP3-RFP

Plasmid

#127558

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

LexGAD

Use

CRISPR

Expression

Insect

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGria2

Plasmid

#124854

Purpose

Mutagenesis of Gria2

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria2 (Gria2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA

Plasmid

#113686

Purpose

An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Publication

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Cre/Lox

Tags

KRAB

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_GATA1_P2A_Hygro_Barcode

Plasmid

#120442

Purpose

Barcoded lentiviral vector to express GATA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Ai133 (TITL-ssAPEX2tm) targeting vector

Plasmid

#114432

Purpose

Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Publication

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ssAPEX2tm

Use

Cre/Lox and Mouse Targeting

Promoter

TREtight

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-KKHSaCas9-BlastR

Plasmid

#107189

Purpose

Confers constitutive expression of KKH SaCas9.

Depositor

Richard Sherwood

Publication

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Insert

KKH SaCas9 (NEWENTRY )

Expression

Bacterial

Mutation

E782K/N968K/R1015H

Promoter

Chicken ß-Actin

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLG1-puro Non-targeting sgRNA 1

Plasmid

#109002

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #1

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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