We narrowed to 29,541 results for: REP

1. mKO2-2A-mTurquoise2

   Plasmid

   #98838

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mKO2-2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31

   Plasmid

   #165887

   Purpose

   G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   All-in-one FC31 RMCE Cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Bmp2

   Plasmid

   #163587

   Purpose

   Expresses BMP-2 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmp2 (Bmp2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR221-mCherry

   Plasmid

   #79509

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   mCherry RFP
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-LifeAct-Venus

   Plasmid

   #87613

   Purpose

   Actin-targeted Venus (YFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-Venus
   

   Use

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-CAG.Pro

   Plasmid

   #79490

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   CAG Promoter
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   CAG Promoter

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330A_D10A-1x7

   Plasmid

   #58777

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO/intron/GFP

   Plasmid

   #113547

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-Bmp5

   Plasmid

   #163589

   Purpose

   Expresses BMP-5 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmp5 (Bmp5 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-ymYPET-CaURA3

    Plasmid

    #118460

    Purpose

    Yeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymYPET
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    YPET A206K, F208S, E232L, N235D

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. sReach-mTurquoise2

    Plasmid

    #98840

    Purpose

    Produces a fusion between mTurquoise2 and sReach. It can be used as a positive control for FRET (using FLIM)

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    sReach-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pR6Kg-Alpha1

    Plasmid

    #165862

    Purpose

    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPB-EF1a-MegaGate-DD-Hygro

    Plasmid

    #175267

    Purpose

    MegaGate destination with C-terminal DD tag and hygromycin resistance

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Tags

    Destabilization Domain

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pISH-NQO1

    Plasmid

    #136766

    Purpose

    Template to synthesize riboprobes for in situ hybridization (ISH) for Nqo1

    Depositor

    Peter Mombaerts

    Article

    Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

    Insert

    Nqo1 (Nqo1 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    ISH Probe insert, V184A- Please see depositor co…

    Promoter

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-EGFP-TARG1

    Plasmid

    #172586

    Purpose

    For transient expression of N-terminal GFP-tagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmTFP1-ShadowG

    Plasmid

    #99867

    Purpose

    Measure FRET between mTFP1 and ShadowG

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    ShadowG
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCZGY2851

    Plasmid

    #87761

    Purpose

    Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Punc-17b-PH-miniSOG(Q103L)
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Promoter

    Punc-17b

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101102

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    TMt-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p.UTA.2.0 Empty

    Plasmid

    #82446

    Purpose

    [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region at the 3'UTR of CFP

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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