We narrowed to 13,038 results for: Mpl

1. pTmpGm

   Plasmid

   #176229

   Purpose

   A template plasmid for amplification of the cI-hok-aacC4 cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, aacC4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-TID-HA3-KanMX6

   Plasmid

   #227980

   Purpose

   To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-MBP-huPb2Cas12a

   Plasmid

   #174686

   Purpose

   Expresses 6xHis-MBP tagged Pb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huPb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMP117

   Plasmid

   #207920

   Purpose

   GreenBraid destination vector pGBZ2 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-CBE6a-IVT

   Plasmid

   #215833

   Purpose

   Template for in vitro transcription of CBE6a (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4xNLS-pMJ915v2-sfGFP

   Plasmid

   #88921

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKDR-EF1a

   Plasmid

   #183567

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated EF1a promoter)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Unattenuated
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-HA-hTAF6

   Plasmid

   #17988

   Depositor

   Naoko Tanese

   Article

   Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

   Insert

   TAF6 (TAF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-YFP-PKD2

   Plasmid

   #31821

   Depositor

   Alexandra Newton

   Article

   Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

   Insert

   YFP-Protein Kinase D2 (PRKD2 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

    Plasmid

    #84251

    Purpose

    Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgCD95-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET14b – lambda exonuclease

    Plasmid

    #104531

    Purpose

    bacterial expression of lambda exonuclease

    Depositor

    Charles Bell

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

    Insert

    λ exonuclease
    

    Use

    Tags

    His6 followed by a thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSK-Sasg

    Plasmid

    #214350

    Purpose

    Expresses cloning backbone for SlugCas9 sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 tracrRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUREF-EX

    Plasmid

    #199331

    Purpose

    A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

    Depositor

    Rikiro Fukunaga

    Article

    Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDD363

    Plasmid

    #91829

    Purpose

    LoxP-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    LoxP-SEC-LoxP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

    Plasmid

    #55217

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE9-His-p97(wt)

    Plasmid

    #14666

    Depositor

    Graham Warren

    Article

    Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

    Insert

    p97 (Vcp Mouse)
    

    Use

    Tags

    RGS6xHis

    Expression

    Bacterial

    Mutation

    See depositor comments below.

    Promoter

    Available since

    April 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 HA Sam68 WT

    Plasmid

    #17690

    Depositor

    David Shalloway

    Article

    Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

    Insert

    Sam68 (Khdrbs1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. LgBit-NbV5

    Plasmid

    #201476

    Purpose

    NanoBit detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    LgBit-NbV5
    

    Use

    Tags

    LgBit

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)

    Plasmid

    #136543

    Purpose

    Expresses ANXA2 chimeras in bacterial cells

    Depositor

    Laszlo Nyitray

    Article

    Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

    Type

    Empty backbone

    Use

    Tags

    ANXA2 (23-339) and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJAT75

    Plasmid

    #204306

    Purpose

    Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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