We narrowed to 17,114 results for: URE

1. pET151-GyrB_T522

   Plasmid

   #216235

   Purpose

   S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

   Depositor

   Eric Brown

   Article

   French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

   Insert

   DNA gyrase
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Changed threonine 522 to isoleucine

   Promoter

   T7

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-Sa-gRNA-puro

   Plasmid

   #191652

   Purpose

   Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   S. aureus gRNAscr
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENT-L1-Kozak-Ark-L2

   Plasmid

   #186362

   Purpose

   Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Ciona robusta Aurora kinase A
   

   Use

   Expression of a fluorescent centrosome marker

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENT-L1-Kozak-HistoneH2B-L2

   Plasmid

   #186357

   Purpose

   Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Histone H2B (H2BC11 Human)
   

   Use

   Expression of a tagged nuclear marker

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

   Plasmid

   #186413

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   lacZ (lacZ E. coli)
   

   Use

   Gateway destination vector

   Tags

   Nuclear localization signal

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST_DHN_SpDHN1 (pBS0799)

   Plasmid

   #185261

   Purpose

   For the mammalian expression of the grass (Stipa purpurea) protein DHN_SpDHN1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DHN_SpDHN1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. L2_NOP1gRNA-Cas9-CsA

   Plasmid

   #136139

   Purpose

   Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CDS12_Cas9-NLS

   Plasmid

   #136122

   Purpose

   Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_AtcoCas9-NLS(noStop)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HBT-CPK32ac-Flag

   Plasmid

   #107431

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK32 (CPK32 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. jam2a-pCRII

    Plasmid

    #68804

    Purpose

    For in situ antisense probe, NotI w/ Sp6

    Depositor

    David Traver

    Article

    Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

    Insert

    jam2a (jam2a Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

11. GoldyTALEN-moesina TAL1

    Plasmid

    #41831

    Depositor

    Jeffrey Essner

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    moesin a (msna Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. GoldyTALEN-moesina TAL2

    Plasmid

    #41832

    Depositor

    Jeffrey Essner

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    moesin a (msna Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSMP-MBD4_3

    Plasmid

    #36376

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD4 (MBD4 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSMP-MBD4_2

    Plasmid

    #36375

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD4 (MBD4 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pGP-CMV-GCaMP6f

    Plasmid

    #40755

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6f
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-GCaMP6s

    Plasmid

    #40753

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6s
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-CMV-GCaMP6m

    Plasmid

    #40754

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6m
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. DYRK1A/pTRE3G-FL-hXIST

    Plasmid

    #149608

    Purpose

    Express doxycycline-inducible full length of human XIST cDNA

    Depositor

    Jeanne Lawrence

    Article

    Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

    Insert

    X-inactive specific transcript (XIST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE3G

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7II-2N4Rtau

    Plasmid

    #177653

    Purpose

    Recombinant protein synthesis of human Tau

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    Tau (2N4R) (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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