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AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

Plasmid

#65458

Purpose

Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

tTA2

Use

AAV

Promoter

CAG

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AMBRA1-3xFLAG

Plasmid

#172605

Purpose

Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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HA-ERalpha

Plasmid

#215093

Purpose

Expresses HA-ERalpha in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

ESR1 (ESR1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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tetO-HAND2

Plasmid

#170690

Purpose

doxycycline-inducible overexpression of HAND2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HAND2 (HAND2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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HA-GR

Plasmid

#215094

Purpose

Expresses HA-tagged Glucocorticoid Receptor in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

NR3C1 (NR3C1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-XRCC1

Plasmid

#215142

Purpose

Expresses mCherry-XRCC1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

XRCC1 (XRCC1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-CDT2

Plasmid

#215091

Purpose

Expresses FLAG-CDT2 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDT2 (DTL Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-POLE1

Plasmid

#215140

Purpose

Expresses mCherry-POLE1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

POLE1 (POLE Human)

Use

Retroviral

Tags

mCherry_FWR

Expression

Mammalian

Mutation

Silent mutations in amino acid 80-86 and 211-217 …

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 blast OFF 3xflag IREB2

Plasmid

#169889

Purpose

Express 3x flag IREB2, suppressible by doxycycline addition

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

IREB2 (IREB2 Human)

Use

Lentiviral; Doxycycline mediated suppression

Tags

3x Flag

Expression

Mammalian

Mutation

Silent mutations in sgIREB2_1 and sgIREB2_2 targe…

Promoter

TRE

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-53BP1

Plasmid

#52507

Purpose

mammalian expression vector of 53BP1

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

V128A and siRNA resistant sequence: ACGCGCTGACGAC…

Promoter

CMV/TO

Available Since

March 26, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

Plasmid

#223965

Purpose

Dual fluorescent reporter for histone H2B and for Cdt1

Depositor

Hee Won Yang

Article

Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

Insert

H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-APEX2

Plasmid

#215127

Purpose

Expresses mCherry-APEX2 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

APEX2 (APEX2 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EMTB-TurboID-V5-puro

Plasmid

#190741

Purpose

A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

EMTB-TurboID-V5 (RPS14 Human, EMTB is human ensconsin; TurboID is engineered BirA from E.coli)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Microtubule binding-domain of ensconsin (amino ac…

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1 KBTBD4 WT

Plasmid

#184624

Purpose

Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

no mutation

Promoter

TRE promoter, Tet ON

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-NR2F1

Plasmid

#170698

Purpose

doxycycline-inducible overexpression of NR2F1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

deletion of 36G- please see depositor comments

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-RSL1D1(WT)

Plasmid

#122301

Purpose

expresses RSL1D1 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RSL1D1 (RSL1D1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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p(HA)HIF1alpha(401delta603)R27G

Plasmid

#52215

Purpose

mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and R27G mutation

Depositor

Eric Huang

Article

Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

Insert

HIF1alpha (401delta603) R27G (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

aa 401-603 deleted, removes the oxygen-dependent …

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
EXO1B-AcGFP

Plasmid

#215125

Purpose

Expresses EXO1B-AcGFP in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

EXO1B (EXO1 Human)

Use

Retroviral

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1859A

Plasmid

#169821

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859A; TCCC -> AGTC silent mutations at nt527…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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mCerulean-PCNA-HygroR

Plasmid

#215064

Purpose

Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Promoter

LTR

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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