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pGEX PLCg1(NC)-SH2

Plasmid

#46471

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(NC) (PLCG1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 542-759

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RAD Module G8-C1

Plasmid

#38208

Depositor

Drew Endy

Publication

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

SET Integrase
Bxb1 Excisonase
RESET Integrase

Use

Synthetic Biology; Biobrick cloning vector

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT PUR mGSK3AWT

Plasmid

#35159

Depositor

Jim Woodgett

Publication

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
F-MED28

Plasmid

#15368

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED28 (MED28 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLEX307-IBV-3CL

Plasmid

#160288

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL

Plasmid

#160284

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBP-split-intein-Gal4[1-20]-N-int

Plasmid

#199201

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[1-20]-N-int

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAd5-B6

Plasmid

#122556

Purpose

Block 6 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Publication

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 25043-30907

Use

Synthetic Biology

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAd5-B4

Plasmid

#122554

Purpose

Block 4 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Publication

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 14475-18018

Use

Synthetic Biology

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAd5-B1-deltaE1-MCS

Plasmid

#122558

Purpose

Modified block 1, with deletion in E1

Depositor

Fred Bunz

Publication

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Mutation

Adenovirus sequences 481-3530 replaced with multi…

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDF_LuxR

Plasmid

#140039

Purpose

Constutitive expression of LuxR protein.

Depositor

Yolanda Schaerli

Publication

Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

Insert

pLuxL and LuxR

Use

Synthetic Biology

Expression

Bacterial

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-LUCI-GECO1

Plasmid

#113675

Purpose

Genetically-encoded ratiometric bioluminescent Ca2+ indicator based on GCaMP6s

Depositor

Robert Campbell

Publication

Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

Insert

LUCI-GECO1

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAGGS-Flag-hsDicer (E1564A)

Plasmid

#41587

Depositor

Phil Sharp

Publication

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

E1564A

Promoter

CMV

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLKO.1 RagD shRNA #2

Plasmid

#30322

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagD shRNA (RRAGD Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

June 2, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1 RagC shRNA #2

Plasmid

#26630

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagC shRNA (RRAGC Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
plk1 WT GFP-myc

Plasmid

#132963

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Publication

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLVX-IRES-Gαq*-BERKY3

Plasmid

#158425

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Use

Lentiviral

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-ncpGCaMP6s

Plasmid

#113674

Purpose

A topological variant of GCaMP6s

Depositor

Robert Campbell

Publication

Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

Insert

ncpGCaMP6s

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLtetO1-deGFP-T500

Plasmid

#45392

Depositor

Vincent Noireaux

Publication

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Expression

Bacterial

Promoter

PL-tetO1

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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