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VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck

Plasmid

#58524

Purpose

Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S, E154A)-TS-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine; changed Glutamate…

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFPV25.1_dGFPmut3-AAV

Plasmid

#187377

Purpose

rpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

Depositor

Roi Avraham

Article

Ciolli Mattioli et al Curr Biol. 2023 Nov 20;33(22):4880-4892.

Insert

dGFPmut3_AAV

Tags

AAV

Expression

Bacterial

Mutation

C-terminal AAV tail

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH2

Plasmid

#183301

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-T43NTR-TrxA-H10

Plasmid

#121677

Purpose

Bacterial expression of neurotensin receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60

Insert

neurotensin receptor (Ntsr1 Rat)

Tags

TrxA-H10

Expression

Bacterial

Mutation

deleted amino acids 1-42

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
pAAV2-GCaMP6f-P2A-Vmn1r86

Plasmid

#133415

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 86

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r86 (Vmn1r86 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP5g-P2A-Vmn1r58

Plasmid

#133418

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 58

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r58 (Vmn1r58 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBA1

Plasmid

#104665

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBA1 (UBA1 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSW618 - pAHP6

Plasmid

#115991

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pAHP6

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSW615 - pATHB-8

Plasmid

#115990

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pATHB-8

Use

Unspecified

Mutation

A1287T to abolish Eco31I site

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pVL15 - tSCR

Plasmid

#116030

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tSCR

Use

Unspecified

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pME18S-3HA-mULK1(K46N)

Plasmid

#22897

Depositor

Masaaki Muramatsu

Article

Yan et al Biochem Biophys Res Commun. 1998 May 8.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Tags

3xHA

Expression

Mammalian

Mutation

changed Lysine 46 to Aspargine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pIAA5::LUC

Plasmid

#141169

Purpose

Luminescent reporter for auxin signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtIAA5 promoter

Use

Luciferase

Expression

Plant

Promoter

AtIAA5, At1g15580

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLMB757

Plasmid

#83792

Purpose

Fructose biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL0489 (frcB) containing all of RL0488 (frcR)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLMB713

Plasmid

#83785

Purpose

Salicylic acid biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL1329 (salA) containing all of RL1328 (salR)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDsRed (GB0100)

Plasmid

#68202

Purpose

Provides the CDS of DsRed as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHB6

Plasmid

#187763

Purpose

MAC-tagged gene expression of human EPHB6

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHB6 (EPHB6 Human)

Expression

Mammalian

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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mOrange2-H2B-6

Plasmid

#57962

Purpose

Localization: Nucleus/Histones, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Insert

H2B (H2BC11 Human)

Tags

mOrange2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pBS-beta1,4 GT-1

Plasmid

#11872

Depositor

Michiko Fukuda

Article

Masri et al Biochem Biophys Res Commun. 1988 Dec 15.

Insert

beta 1,4 galactosyltransferase (B4GALT1 Human)

Expression

Bacterial

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
SLF091: Lenti-CaMKIIa-eTsChR-eGFP

Plasmid

#112281

Purpose

For lentiviral or direct expression of eTsChR in excitatory neurons

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

eTsChR

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-6His-MyoX

Plasmid

#47607

Depositor

Emanuel Strehler

Article

Bennett et al Biochem Biophys Res Commun. 2008 May 2;3

Insert

MyoX (MYO10 Human)

Expression

Mammalian

Mutation

Q680R

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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