We narrowed to 22,070 results for: ESC

1. Nanog-2A-mCherry

   Plasmid

   #59995

   Purpose

   Inserts mCherry immediately downstream of the Nanog coding region

   Depositor

   Rudolf Jaenisch

   Article

   Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2A-mCherry

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nanog-2A-GFP

   Plasmid

   #59994

   Purpose

   Inserts GFP immediately downstream of the Nanog coding region

   Depositor

   Rudolf Jaenisch

   Article

   Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2A-GFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNANOG-Luc

   Plasmid

   #25900

   Depositor

   Ren-he Xu

   Article

   Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

   Insert

   NANOG promoter (NANOG Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL2- Empty control -SV40-Luc

   Plasmid

   #26280

   Purpose

   Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

   Depositor

   Rudolf Jaenisch

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-TIGHT-EGFP-backward donor

   Plasmid

   #22077

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   TETO-eGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBRPyCAG-hKlf2-DsRed-Ip

   Plasmid

   #26277

   Depositor

   Rudolf Jaenisch

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

   Insert

   Klf2 (KLF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1 SA-2A-puro-pA donor

   Plasmid

   #22075

   Purpose

   AAV zinc finger donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   SA-Puro
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1 hPGK-PuroR-pA donor

   Plasmid

   #22072

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   PGK-Puro (PGK1 Human)
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CAGGS-EGFP

   Plasmid

   #22212

   Purpose

   AAV zinc finger donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   CAGGS-eGFP
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE-TIGHT-EGFP-donor fw copy

    Plasmid

    #22074

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    TETO-eGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFrt-invCAG-ires-Luc

    Plasmid

    #63576

    Purpose

    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.

    Depositor

    Ivo Huijbers

    Article

    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

    Insert

    Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
    

    Use

    Cre/Lox; Frt/flpe recombination mediated insertion

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCol1a1-frt (FVB)

    Plasmid

    #63575

    Purpose

    Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.

    Depositor

    Ivo Huijbers

    Article

    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

    Insert

    Col1A-frt-hygro-pA
    

    Use

    Mouse Targeting; Frt/flpe

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    None

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-tetpA-hNEUROG2-iRPT

    Plasmid

    #140764

    Purpose

    Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

    Inserts

    iRFP713
    PuroR
    rtTAM2
    NEUROG2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBRPyCAG-fmKlf4-DsRed-Ip

    Plasmid

    #26274

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sox2-SE-RGM-eGFP targeting vector

    Plasmid

    #133045

    Purpose

    Targeting vector of RGM-eGFP with homologous arms around Sox2 super-enhancer DMR

    Depositor

    Rudolf Jaenisch

    Article

    Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

    Insert

    Sox2-SE-5arm-RGM-Sox2-SE-3arm
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFrt-invCAG-Luc

    Plasmid

    #63577

    Purpose

    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites

    Depositor

    Ivo Huijbers

    Article

    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

    Insert

    Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
    

    Use

    Cre/Lox; Frt/flpe recombination mediated insertion

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. miR290-SE-RGM-eGFP targeting vector

    Plasmid

    #133046

    Purpose

    Targeting vector of RGM-eGFP with homologous arms around Mir290 super-enhancer DMR

    Depositor

    Rudolf Jaenisch

    Article

    Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

    Insert

    miR290-SE-5arm-RGM-miR290-SE-3arm
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-Ctr1-gRNA-EGFP

    Plasmid

    #226000

    Purpose

    Negative control for CRISPRi-KD

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    negtive control sgRNA of no specific targets in human genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-Ctr2-gRNA-EGFP

    Plasmid

    #226001

    Purpose

    Negative control for CRISPRi-KD

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    negtive control sgRNA of no specific targets in human genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-hL1-gRNA1-EGFP

    Plasmid

    #226002

    Purpose

    CRISPRi-KD of human LINE1

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    sgRNA targeting human L1HS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge