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Plasmid#48748PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationMutated E-box2 sequence from CACGTT to GCTAGTPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-p62 PB1mut
Plasmid#204577PurposeExpresses 3xFLAG-p62 PB1mut in mammalian cellsDepositorInsertp62 (Sqstm1 Mouse)
UseTagsExpressionMammalianMutationK7A/D69APromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-fullef1a-mutFAKSPARK2
Plasmid#195266Purposeexpress Tyr to Phe mutant FAKSPARK2 in a lenti vectorDepositorInsertTyr to Phe mutant FAKSPARK2 sensor
UseLentiviralTagsExpressionMammalianMutationPromoteref1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6 mut AP-1+C/EBP
Plasmid#61292Purposedrives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sitesDepositorInsertIL-6 promoter (Il6 Mouse)
UseLuciferaseTagsExpressionMammalianMutationMutated both AP-1 binding site from TGAGTCT to TG…PromoterIL-6 promoter with mutant AP-1 and C/EBP binding …Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pCadBA-mCherry
Plasmid#192865PurposepMUT2 based vector for sensing pH in E. coli Nissle 1917DepositorInsertpH sensitive promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDae029 (eMutaT7[TadA-8e])
Plasmid#187620PurposeExpresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertTadA-8e-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pTcpP-mCherry
Plasmid#192861PurposepMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917DepositorInsertTcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NEmut
Plasmid#123310PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE control sensor GRAB_NEmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CDMut
Plasmid#101920PurposeThe dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.DepositorInsertdCas9-TET1CD Mut
UseLentiviralTagsExpressionMammalianMutationMutated TET1 catalytic domainPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-PA-mCherry
Plasmid#138251PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-PAmCherry
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_HA1mut
Plasmid#190475PurposeExpress the histamine-insensitive control sensor GRAB_HA1mut in neuronsDepositorInsertHistamine-insensitive sensor GRAB_HA1mut (control sensor)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pLac-mCherry
Plasmid#192859PurposepMUT2 based vector for sensing lactic acid in E. coli Nissle 1917DepositorInsertlldR operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+/mit-2mutAEQ
Plasmid#45539DepositorInsertmit-2mutAEQ
UseTagsMitochondrially targetedExpressionMammalianMutationAsp119Ala and Asn28LeuPromoterAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_ATP1.0mut
Plasmid#167578PurposeExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in neuronsDepositorInsertATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_eCBmut
Plasmid#164605PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCBmut in neuronsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseAAVTagsExpressionMutationS383T, F177APromoterhSynAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_Ado1.0mut
Plasmid#153290PurposeExpress the adenosine-insensitive control sensor GRAB_Ado1.0mut in astrocytesDepositorInsertAdenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTHmut
Plasmid#200159PurposeFor bacterial expression of the APOBEC1-YTHmut fusion protein for protein purificationDepositorInsertAPOBEC1-YTHmut
UseTags6xHis, HA, and MBPExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I T7
Plasmid#208191Purposeframeshift mistranslation, T7DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6TTTTTTTPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I A5
Plasmid#208192Purposeframeshift mistranslation, A5DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6AAAAAPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only