We narrowed to 9,000 results for: Pol;

1. pCMV6-AN-DDK_3KA POLI

   Plasmid

   #131239

   Purpose

   Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert lysine resi…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGST-pol1-N2-2A2

   Plasmid

   #160991

   Purpose

   Express a fragment of yeast pol1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   pol1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F58A/D62A

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGST-pol1-N1-2A2

   Plasmid

   #160990

   Purpose

   Express a fragment of yeast pol1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   pol1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F58A/D62A

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27-Linear-1_PolyU(1)

   Plasmid

   #159479

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV-U6+27-Linear-1_PolyU(2)

   Plasmid

   #159480

   Purpose

   Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV-U6+27-Linear-1_PolyU(3)

   Plasmid

   #159481

   Purpose

   Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmiRGLO-POLR2E-213

   Plasmid

   #117356

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR POLR2E-213
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. YCe1889 HC_Kan_SV40-polyA_p11

   Plasmid

   #100678

   Purpose

   part designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequence

   Depositor

   Yizhi Cai

   Article

   Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

   Insert

   SV40-polyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

   Plasmid

   #168290

   Purpose

   CRISPR-activator Suntag-P65-HSF1 (SPH)

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   dCas9, Suntag-P65-HSF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. v5 eVLP gag-pro-pol

    Plasmid

    #228466

    Purpose

    Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag-pro-pol(Q226P)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q226P

    Promoter

    CMV

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAH-POLY-eRF1(E55D)

    Plasmid

    #157925

    Purpose

    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

    Insert

    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV uN2CpolyGly op GFP (NIID)

    Plasmid

    #224356

    Purpose

    Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)

    Depositor

    Nicolas Charlet-Berguerand

    Article

    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

    Insert

    uN2C with a GGC repeat expansion (100x)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Codon-optimized for expression in human, so no pu…

    Promoter

    CMV + chimeric intron (CAG)

    Available since

    Feb. 5, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pEvolvR-enCas9-PolI3M-TBD

    Plasmid

    #113077

    Purpose

    Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber, David Schaffer

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI3M-TBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)

    Plasmid

    #195552

    Purpose

    Expresses Thbs1 under control of short GFAP promoter

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    short GFAP

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA pDEST40 Cas9-POLD3

    Plasmid

    #183194

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    POLD3
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid

    #229679

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid

    #112285

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-Flag-H3.3-polyA

    Plasmid

    #59780

    Purpose

    Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    H3F3B (H3-3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFUGW-Pol2-ffLuc2-eGFP

    Plasmid

    #71394

    Purpose

    Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

    Depositor

    Glenn Merlino

    Article

    Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

    Insert

    Pol2-ffLuc2-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pol2 (mouse RNA polymerase II gene promtoer)

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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