We narrowed to 9,173 results for: Pol;

1. pCMV6-AN-DDK_S497A POLI

   Plasmid

   #131236

   Purpose

   Mammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a point mutation converting Pol iota ser…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_S520A POLI

   Plasmid

   #131237

   Purpose

   Mammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a point mutation converting Pol iota ser…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-DDK_S580A POLI

   Plasmid

   #131238

   Purpose

   Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a point mutation converting Pol iota ser…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-AN-DDK_3KA POLI

   Plasmid

   #131239

   Purpose

   Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert lysine resi…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGST-pol1-N2-2A2

   Plasmid

   #160991

   Purpose

   Express a fragment of yeast pol1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   pol1
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F58A/D62A

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGST-pol1-N1-2A2

   Plasmid

   #160990

   Purpose

   Express a fragment of yeast pol1 protein

   Depositor

   Zhiguo Zhang

   Article

   Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

   Insert

   pol1
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F58A/D62A

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV-U6+27-Linear-1_PolyU(1)

   Plasmid

   #159479

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(1)
   

   Expression

   Mammalian

   Promoter

   U6-27

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV-U6+27-Linear-1_PolyU(2)

   Plasmid

   #159480

   Purpose

   Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(2)
   

   Expression

   Mammalian

   Promoter

   U6-27

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV-U6+27-Linear-1_PolyU(3)

   Plasmid

   #159481

   Purpose

   Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(3)
   

   Expression

   Mammalian

   Promoter

   U6-27

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiRGLO-POLR2E-213

    Plasmid

    #117356

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR POLR2E-213
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Murine PGK-1

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. YCe1889 HC_Kan_SV40-polyA_p11

    Plasmid

    #100678

    Purpose

    part designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequence

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    SV40-polyA
    

    Use

    Synthetic Biology

    Available Since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid

    #229679

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid

    #229680

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    SNAP, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AP1muA-TurboID-polyA-G418 HR

    Plasmid

    #227734

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. v5 eVLP gag-pro-pol

    Plasmid

    #228466

    Purpose

    Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag-pro-pol(Q226P)
    

    Expression

    Mammalian

    Mutation

    Q226P

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1-FHC-POLQ

    Plasmid

    #64875

    Purpose

    lentiviral expression of human POLQ

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEvolvR-enCas9-PolI3M-TBD

    Plasmid

    #113077

    Purpose

    Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI3M-TBD
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFUGW-Pol2-ffLuc2-eGFP

    Plasmid

    #71394

    Purpose

    Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

    Depositor

    Glenn Merlino

    Article

    Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

    Insert

    Pol2-ffLuc2-eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Pol2 (mouse RNA polymerase II gene promtoer)

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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