We narrowed to 457 results for: Si

1. PB-TAC-Cas9

   Plasmid

   #120352

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on and CBh

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-KRAB-dCas9

   Plasmid

   #120355

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on and CBh

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBEL2082

   Plasmid

   #155162

   Purpose

   Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   (MlaFΔ[248-269])-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids 248-269 in MlaF

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEL1514

   Plasmid

   #155147

   Purpose

   Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEL1965

   Plasmid

   #155152

   Purpose

   Expresses MlaF(1-246)-(6 aa linker)-GCN4

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   mlaF(1-246)-GCN4_Dimerization_Domain
   

   Use

   Tags

   Expression

   Mutation

   deleted amino acids 247-269 in MlaF; added dimeri…

   Promoter

   araBAD

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEL1966

   Plasmid

   #155153

   Purpose

   Expresses MlaF(1-246)-(10 aa linker)-GCN4

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   mlaF(1-246)-GCN4_Dimerization_Domain
   

   Use

   Tags

   Expression

   Mutation

   deleted amino acids 247-269 in MlaF; added dimeri…

   Promoter

   araBAD

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEL1967

   Plasmid

   #155154

   Purpose

   Expresses MlaF(1-246)-(14 aa linker)-GCN4

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   mlaF(1-246)-GCN4_Dimerization_Domain
   

   Use

   Tags

   Expression

   Mutation

   deleted amino acids 247-269 in MlaF; added dimeri…

   Promoter

   araBAD

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CAG-Avr-HPRT-RIGHT

   Plasmid

   #107272

   Purpose

   Expresses TALEN (right) targeting HPRT exon3

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   HPRT1_B Avr-TALEN (right)
   

   Use

   TALEN

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-Avr-HPRT-LEFT

   Plasmid

   #107271

   Purpose

   Expresses TALEN (left) targeting HPRT exon3

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   HPRT1_B Avr-TALEN (left)
   

   Use

   TALEN

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVS1-P-MCS

    Plasmid

    #80488

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-EFS-PEmax-P2A-Puro

    Plasmid

    #228890

    Purpose

    Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Inserts

    PEmax
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYESir2HY-puro

    Plasmid

    #1770

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H363Y, residue 363 changed from histidine to tyro…

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTALdNC-AAVS1_T1

    Plasmid

    #80495

    Purpose

    AAVS1 TALEN expression vector (Left)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Left)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTALdNC-AAVS1_T2

    Plasmid

    #80496

    Purpose

    AAVS1 TALEN expression vector (Right)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Right)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-P-CAG-DEST

    Plasmid

    #80490

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAVS1-Nst-MCS

    Plasmid

    #80487

    Purpose

    donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMMB67EH-Spy

    Plasmid

    #90102

    Purpose

    IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

    Depositor

    Samuel Miller

    Article

    Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

    Insert

    YcgR (ycgR )
    

    Use

    Broad-host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD-GINKO1

    Plasmid

    #113111

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Use

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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