We narrowed to 9,374 results for: App

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pPP076

Plasmid

#158794

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-1

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pShuttle mCherry-Tubulin

Plasmid

#26768

Depositor

Torsten Wittmann

Article

Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
pCAG-Ace2_D92N_E199V-mNeon

Plasmid

#129256

Purpose

Express Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pWS5399-CRISPRai-ZeoR

Plasmid

#182838

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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GEV 2

Plasmid

#12616

Depositor

John Louis

Article

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)

Tags

G protein and His

Expression

Bacterial

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pME-GFP-P2A

Plasmid

#80807

Purpose

GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-P2A

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pME-Ruby2-NS

Plasmid

#166569

Purpose

Multisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Ruby2

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-BASU-EGFP

Plasmid

#163845

Purpose

In vitro transcription of BASU-EGFP. Parton lab clone JRR

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

BASU

Use

In vitro transcription of rna

Tags

EGFP

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pST-Ki

Plasmid

#44563

Purpose

for integrative constitutive expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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Degron-KI SF3B1 donor vector

Plasmid

#65622

Purpose

Donor vector to generate Degron-KI DD-SF3B1

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

SF3B1 Degron-KI left and right HR arms

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-b3STOPb3-ZsGreen

Plasmid

#51266

Purpose

B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

b3STOPb3-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-Positron-ST

Plasmid

#129254

Purpose

Express soma-localized Positron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Gq sensor

Plasmid

#137810

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid

Depositor

Dorus Gadella

Article

Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

Insert

Gq sensor

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAdEasy mCherry-Tubulin

Plasmid

#26767

Depositor

Torsten Wittmann

Article

Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Golden-Gate-Esp3I-Destination

Plasmid

#51278

Purpose

CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFUM

Plasmid

#133130

Purpose

A recombinant plasmid expressing fum (fumarase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

fumarase gene

Expression

Bacterial

Promoter

PEP-carboxykinase promoter

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMUT2-pLac-mCherry

Plasmid

#192859

Purpose

pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

lldR operon

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-mKate2 no-pA

Plasmid

#80828

Purpose

P2A followed by mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-mKate2 no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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UAS::TVA

Plasmid

#164487

Purpose

Expression of TVA

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Insert

TVA (CD320 Chicken)

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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