We narrowed to 36,658 results for: ELL

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pcDNA3.1+_hsTRPA1_myc_dTom

Plasmid

#183179

Purpose

myc tagged hsTRPA1 used for cell transfection

Depositor

Sreekanth Chalasani

Article

Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

Inserts

hsTRPA1 (TRPA1 Human)
dTom

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a-ValRS-His

Plasmid

#124122

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Valyl-tRNA ligase (valS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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AICSDP-42:AAVS1-mTagRFPT-CAAX

Plasmid

#107580

Purpose

Homology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cells

Depositor

Allen Institute for Cell Science

Insert

AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

May 9, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pQCXIH-Myc-YAP-S94A

Plasmid

#33094

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S94A

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pLGB31

Plasmid

#126621

Purpose

Suicide vector for allelic replacement in Bacteroides, inulin selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NFIA+SOX9

Plasmid

#129455

Purpose

Inducible overexpression of NFIA and Sox9 in human cells.

Depositor

Su-Chun Zhang

Article

Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

Insert

Sox 9 (SOX9 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-BRCA1

Plasmid

#115491

Purpose

EGFP-BRCA1 expression vector. Parton clone BXY

Depositor

Rob Parton

Article

McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

Insert

BRCA1 (BRCA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S1009R (See depositor comments)

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMK345 (Hygro-P2A-mAID-mClover)

Plasmid

#121179

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-mClover

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MG-155

Plasmid

#26529

Depositor

David Baltimore

Article

O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Insert

miR-155 (MIR155 Human)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pET23-CBD-(-PP)-EGFP

Plasmid

#12597

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

Cdc42 Biosensor (WAS Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

F271C

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pYFJ16-LplA(W37V) (for E. Coli expression)

Plasmid

#34838

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA W37V (lplA E. Coli)

Tags

His Tag

Expression

Bacterial

Mutation

Mutated Trp37 to Val

Promoter

T5 promoter

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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FRE5

Plasmid

#62377

Purpose

Fluorescent reporter to quantify the ratio of two alternative splicing patterns.

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209722

Purpose

Expresses mitochondrially targeted low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pSicoR human DGCR8-1

Plasmid

#14769

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-R-Creon shRNA[Control]

Plasmid

#180775

Purpose

This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBJ FLAG-hPar1

Plasmid

#53226

Purpose

mammalian expression of human Par1 with N-terminal FLAG tag

Depositor

Shaun Coughlin

Article

Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

Insert

Par1 (F2R Human)

Tags

FLAG and bovine prolactin signal sequence (MDSKGS…

Expression

Mammalian

Mutation

Deletion of aa1-39 (native signal peptide)

Promoter

SV40

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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FR_HNF1B

Plasmid

#31101

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

wild type

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

Plasmid

#85756

Purpose

All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

Depositor

Lawrence Stanton

Article

Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

Inserts

Csy4-T2A-SpRFN-P2A-GFP
gRNA

Use

CRISPR

Tags

Csy4, Csy4 recognition sequence, FokI fusion via …

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV and U6

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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PB53x EF1-Dendra2-RPB1Amr

Plasmid

#81228

Purpose

expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system

Depositor

Timothée Lionnet

Article

Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1

Insert

Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)

Tags

Dendra2

Expression

Mammalian

Mutation

N792D alpha amanitin resistance mutation (Bartolo…

Promoter

EF1a

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pU6_gRNA_handle_U6t

Plasmid

#49016

Purpose

gRNA expression empty vector (mammalian cells)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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