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pemiRFP703-N1

Plasmid

#136558

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE-Slow-FT

Plasmid

#31915

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Slow-FT

Expression

Mammalian

Mutation

M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

Promoter

PTight Tet-responsive PminCMVd

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pTRE-Fast-FT

Plasmid

#31913

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Expression

Mammalian

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

PTight Tet-responsive PminCMVd

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pTRE-Medium-FT

Plasmid

#31914

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

PTight Tet-responsive PminCMVd

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eNpHR-EYFP-WPRE

Plasmid

#20946

Purpose

3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

halorhodopsin

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only

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pmiRFP720-N1

Plasmid

#136560

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP720

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Lifeact-mScarlet3-S2

Plasmid

#246873

Purpose

Expression of mScarelt3-S2 fused with Lifeact. This construct is used for imaging actin filaments.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

Lifeact

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#167547

Purpose

Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

Depositor

Howard Hang

Article

Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

Inserts

chloramphenicol acetyl transferase
pUC19 origin of replication

Use

CRISPR

Expression

Bacterial

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pKG2440_pGEEC536_pShip-UbC-mRuby2-P2A-PuroR-bGH

Plasmid

#239732

Purpose

Plasmid expressing mRuby2 and PuroR with the UbC promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD1

Plasmid

#153116

Purpose

Expression of Flag tagged human FOXD1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD1 (FOXD1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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mScarlet3-S2-Sec61β

Plasmid

#246874

Purpose

Expression of mScarelt3-S2 fused with Sec61β. This construct is used for imaging the ER.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

Sec61β

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pK18sB

Plasmid

#177838

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

Depositor

Gregg Beckham, Christopher Johnson

Article

Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Type

Empty backbone

Use

Bacterial gene replacement

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-FOXA1

Plasmid

#153109

Purpose

Expression of Flag tagged human FOXA1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXA1 (FOXA1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXK1

Plasmid

#153132

Purpose

Expression of Flag tagged human FOXK1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXK1 (FOXK1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXL1

Plasmid

#153134

Purpose

Expression of Flag tagged human FOXL1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXL1 (FOXL1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD2

Plasmid

#153117

Purpose

Expression of Flag tagged human FOXD2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD2 (FOXD2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXS1

Plasmid

#153152

Purpose

Expression of Flag tagged human FOXS1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXS1 (FOXS1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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CD9-mEGFP

Plasmid

#182864

Purpose

Localization: Tetraspanin/Membrane

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

human CD9 (CD9 Human)

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGLuc

Plasmid

#135288

Purpose

GBPart - GLuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Gaussia Luciferase

Use

Luciferase and Synthetic Biology

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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LifeAct-MTED

Plasmid

#217498

Purpose

Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

Depositor

Erik Dent

Article

Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

Insert

LifeAct-mScarlet-MTED

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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