We narrowed to 3,862 results for: SPL;

1. pCDNA3_altPKA-Cα-mPAGFP

   Plasmid

   #114133

   Purpose

   Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   Testis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN-iRS3GG

   Plasmid

   #98858

   Purpose

   Expresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

   Depositor

   Lydia Contreras

   Article

   Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

   Insert

   PheS cassette to be selectively replaced by unique asRNA probes
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-DmMextli

   Plasmid

   #79262

   Purpose

   Expresses EGFP-tagged DmMextli in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

   Insert

   DmMextli (mxt Fly, NM_164598)
   

   Use

   Schneider 2 cell expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Ac5 promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-hPMCA2x/b delta6

   Plasmid

   #47587

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2x/b delta6 (ATP2B2 Human)
   

   Use

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Mutation

   6 C-terminal AA deleted (needed for PDZ binding)

   Promoter

   CMV

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pRP.ATM c.7865C>T

   Plasmid

   #226554

   Purpose

   Minigene encoding the ATM c.7865C>T mutation

   Depositor

   Dave Wee

   Article

   Tommaso et al Molecular Therapy: Nucleic Acid (2025)

   Insert

   ATM (ATM Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   c.7865C>T

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_RTN3E-mScarlet3

   Plasmid

   #216255

   Purpose

   mammalian expression vector for RTN3E-mScarlet3

   Depositor

   Edward Avezov

   Article

   Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

   Insert

   reticulon 3 isoform e (RTN3 Human)
   

   Use

   Tags

   mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. L2379-FKBP-GPA(V84R)-20-11S

   Plasmid

   #222901

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA(V84R)-20GS-NanoLuc11S
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. L2380-FKBP-GPA(V84R)-20-114

   Plasmid

   #222900

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA(V84R)-20GS-NanoLuc114
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. L2378-FRB-GPA(V84R)-20-11S

   Plasmid

   #222899

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(V84R)-20GS-NanoLuc11S
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L2377-FRB-GPA(V84R)-20-114

    Plasmid

    #222898

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-20GS-NanoLuc114
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAL-MBP-FRB-Cdc42/N12

    Plasmid

    #214290

    Purpose

    Express split-Cdc42 fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Cdc42 fragment fused with FRB (CDC42 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21b-Cdc42/13C-FKBP-MBP

    Plasmid

    #214291

    Purpose

    Express split-Cdc42 fragment fused with FKBP and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Cdc42 fragment fused with FKBP (CDC42 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Cdc42-Q61L

    Promoter

    T7

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAL-MBP-FRB-Rac1/N12

    Plasmid

    #214294

    Purpose

    Express split-Rac1 fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Rac1 fragment fused with FRB (RAC1 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b-Rac1/13C-FKBP-MBP

    Plasmid

    #214295

    Purpose

    Express split-Rac1 fragment fused with FKBP and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Rac1 fragment fused with FKBP (RAC1 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Rac1-Q61L

    Promoter

    T7

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMAL-MBP-FRB-RhoA/N12

    Plasmid

    #214298

    Purpose

    Express split-RhoA fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-RhoA fragment fused with FRB (RHOA Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21b-RhoA/13C-FKBP-MBP

    Plasmid

    #214299

    Purpose

    Express split-RhoA fragment fused with FKBP and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-RhoA fragment fused with FKBP (RHOA Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    RhoA-Q63L

    Promoter

    T7

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

    Plasmid

    #213728

    Purpose

    Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

    Plasmid

    #202090

    Purpose

    Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

    Depositor

    Elizabeth Heller

    Article

    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

    Insert

    dCas9-Set2(R195G, C201A)
    

    Use

    Tags

    3x FLAG and mCherry

    Expression

    Mammalian

    Mutation

    R195G and C201A abolish catalytic activity

    Promoter

    human Synapsin

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)

    Plasmid

    #209776

    Purpose

    CRISPRi for STK4

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    Nluc, sgSTK4i, mRuby2 (STK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR)

    Plasmid

    #209773

    Purpose

    CRISPRi for STK4

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    dCas9-KRAB, sgSTK4i (STK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge