We narrowed to 31,012 results for: uros
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Plasmid#190485PurposeMammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.DepositorInsertPan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro SPI1 P2A mCherry
Plasmid#140029PurposeTunable and temporal expression control of SPI1 and mCherryDepositorInsertSPI1 (SPI1 Human)
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro PFKM
Plasmid#201638PurposeOver-expression of untagged human PFKMDepositorInsertPFKM (PFKM Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 3
Plasmid#109003PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #3
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
1272 pBabe puroL Myr HA Akt3
Plasmid#9019DepositorInsertAkt3 (AKT3 Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-GFP-miRE_shRen-Puro
Plasmid#135683PurposeConstitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)_v2
Plasmid#74408PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, Human, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc
Plasmid#161584Purposeexpression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S mutationDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationD1994SPromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro GSK3 beta (S9A)
Plasmid#14128DepositorInsertGSK3 beta (S9A) (GSK3B Human)
UseRetroviralTagsV5 tagExpressionMammalianMutationS9A. Mutant version of GSK3 that lacks the inhibi…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAc5 HA3-eDHFR-T2A-puro
Plasmid#86395Purposefor C-terminal tagging with eDHFR trimethoprim regulated degron with puro selectionDepositorInsertHA3 eDHFR T2A puro
UseCRISPRTagsHA3 eDHFR T2A puroExpressionMutationR12Y, G67S and Y100I mutations in E coli DHFRPromoterAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-HA-AU1
Plasmid#162107PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-HA-AU1
UseLentiviralTagsVSVg-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-FLAG
Plasmid#162112PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to StrepTagII-HA-FLAG
UseLentiviralTagsStrepTagII-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-HA
Plasmid#162089PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to StrepTagII-ProtC-HA
UseLentiviralTagsStrepTagII-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-HA
Plasmid#162080PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-StrepTagII-HA
UseLentiviralTagsVSVg-StrepTagII-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-mgp100-PuroR [M1G]
Plasmid#171802PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-mgp100-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR
Plasmid#162335PurposeLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shSCR-Puro
Plasmid#135689PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selectionDepositorInsertshScrambled
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)
Plasmid#139448PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 N192Q/N200Q
Plasmid#121453Purposeexpression of human PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationN192Q/N219QPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRASQ61K-IRES-Puro
Plasmid#120570PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61KPromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only