We narrowed to 51,827 results for: ANG

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KBB418

Plasmid

#185088

Purpose

Swi6L-GFP with nuclear export signal mutated to alanines

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 nuclear export signal L to A mutations

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWR

Plasmid

#112900

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ133-RZ-Cas12j2

Plasmid

#189784

Purpose

Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-FAID

Plasmid

#127885

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID (PPP2CA Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT6-G60A-FLAG

Plasmid

#102323

Purpose

Human SIRT6 G60A with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed glycine 60 to alanine

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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th-P2A-Gal4 donor

Plasmid

#65563

Purpose

Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-Gal4 (th Zebrafish)

Use

Zebrafish injection

Promoter

none

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pMT462

Plasmid

#139393

Purpose

XOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

XOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs

Use

Synthetic Biology

Promoter

aTC and BA inducible promoters

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NESM

Plasmid

#13282

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pYPQ290

Plasmid

#129670

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NLS1D

Plasmid

#13284

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa134-aa142

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pYPQ292

Plasmid

#129672

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NLS1/2D

Plasmid

#13287

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa134-aa142, aa150-aa155

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
GFP-NPM NESM-NLS1D

Plasmid

#13288

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A, deletion of aa134-aa142

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pYPQ141-ZmUbi-RZ-Cas12j2

Plasmid

#173926

Purpose

Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NESD

Plasmid

#13283

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa94-aa102

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pdCas9-PBE

Plasmid

#98161

Purpose

Expresses CRISPR base editor in protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Expression

Plant

Promoter

Ubi

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-th-sgRNA

Plasmid

#65565

Purpose

in vitro trancription of sgRNA targeting the zebrafish th locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th sgRNA (th Zebrafish)

Use

In vitro rna transcription

Promoter

T7

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pB-Hygro-rKIF5B-GFP

Plasmid

#210335

Purpose

Overexpression of rKIF5B

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rKIF5B (Kif5b Rat)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWC

Plasmid

#112898

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ-Cas12j2

Plasmid

#173924

Purpose

Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

NLS-Cas12j2-NLS

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWY

Plasmid

#112901

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-NPM NLS2D

Plasmid

#13285

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa150-aa155

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#164046

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mTagBFG-TriTag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 AT1R DRY-AAY

Plasmid

#45759

Depositor

Laszlo Hunyady, Robert Lefkowitz

Article

Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

Insert

AT1R DRY-AAY (Agtr1a Rat)

Tags

HA

Expression

Mammalian

Mutation

changed amino acids 125-126 from DR to AA

Promoter

CMV

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pTPI1-EMC1-GFP-APEX2-URA3

Plasmid

#184776

Purpose

Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

APEX2

Expression

Yeast

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ239A (dFnCas12a)-TV

Plasmid

#136379

Purpose

CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

dFnCas12a-TV

Use

CRISPR

Expression

Plant

Mutation

D917A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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LDB204

Plasmid

#185426

Purpose

Bacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 FXFG-repeats domain expressed

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-CDS1-3HA

Plasmid

#198596

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-DGK1-3HA

Plasmid

#198595

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-PAH1-3HA

Plasmid

#198597

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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