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pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100849

Purpose

AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

Plasmid

#100846

Purpose

AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRB-V5/HIS

Plasmid

#201988

Purpose

expression of human PDGFRB receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRB receptor tyrosine kinase (PDGFRB Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_SNCA:EGFP

Plasmid

#92195

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-SNCA-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-RET-V5/HIS

Plasmid

#205606

Purpose

Expression of human RET receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

RET receptor tyrosine kinase (RET Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Lamp1-mCherry-FRB

Plasmid

#186576

Purpose

Expression of mCherry-FRB at the outer surface of the lysosome using LAMP1

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

LAMP1 (LAMP1 Human)

Tags

mCherry-FRB

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAC150-RFP-EGFP-LGALS3

Plasmid

#175778

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-LGALS3

Plasmid

#175756

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXW109Hg(RS-RinA-E11)2

Plasmid

#123151

Purpose

Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT, PhrpLE, PrinA

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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palm-Bassoon-GFP

Plasmid

#250161

Purpose

Encodes a version of rat Bassoon with a C-terminal GFP-tag and with a palmitoylation consensus replacing the N-terminal 94 aa. For mammalian expression.

Depositor

Thomas Dresbach

Article

Kapoor et al Elife. 2026 Jan 13;13:RP98254. doi: 10.7

Insert

palmitoylated Bassoon-GFP construct (N-terminal 94 aa replaced by palmitoylation consensus) (Bsn Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100855

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRKC-V5/HIS

Plasmid

#205615

Purpose

Expression of human TRKC receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TRKC receptor tyrosine kinase (NTRK3 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCB’ CbbL Y72R

Plasmid

#162707

Purpose

Carboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomes

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Mutation

CbbL Y72R; Substitution of CsoS2-binding tyrosine…

Promoter

PLtet0-1 promoter

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-GCaMP7f

Plasmid

#135539

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

GCaMP7f

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100851

Purpose

AAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_MAPT:EGFP

Plasmid

#92196

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for Tau screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-MAPT-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pKrox24(2xD-E_inD)Luc

Plasmid

#200109

Purpose

Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element in front of EGR1 promoter…

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5D-FLAG

Plasmid

#72197

Purpose

Expresses full-length Unc5d with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d (Unc5d Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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p8327 pLIX YAP1 S127A

Plasmid

#184528

Purpose

Expression of YAP1 S127A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S127A (YAP1 Human)

Use

Lentiviral

Mutation

S127A

Promoter

TRE promoter, Tet ON

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLX317-QKI

Plasmid

#115441

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

QKI (QKI Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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