We narrowed to 14,439 results for: eng
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Plasmid#223249PurposeMammalian expression of the optimized R2Tg RNA donorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertR2Tg donor_opt
ExpressionMammalianAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-PH(Akt2)
Plasmid#86596PurposeExpresses mCherry-tagged PH(WT) domain of human Akt2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAkt2 PH domain (AKT2 Human)
ExpressionMammalianMutationAmino acids 1 - 108Available sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EGFR-V5/HIS
Plasmid#201102Purposeexpression of human EGFR receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)
TagsV5/HisExpressionMammalianPromoterCMVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB R73Q
Plasmid#60420PurposeMammalian Expression of ITSN DH/PH-tgRFPt-MicroDepositorInserthITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)
UseCre/Lox and LentiviralTagstgRFPtExpressionMammalianMutationhITSN 1159-1509 // SSPB R73Q, Y11K and A15EPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPR v2 sgKEAP1-2
Plasmid#186459Purposeknock out KEAP1 in mammalian cellsDepositorInsertKeap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
UseCRISPR and LentiviralAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGT-Ah1
Plasmid#122568PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN7HELIX
Plasmid#190662PurposeExpresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
ExpressionMammalianMutationnAniI = K227M, F80K, L232KPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_8xMmaPylT_EF1_FLAG-MmaPylRS
Plasmid#174890PurposeMma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationU25C PylTPromoterEF1Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAR18_StrepI_mFASm_H8_pET22b
Plasmid#122847PurposeExpresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertmouse FASN/ mouse fatty acid synthase (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cerulean-2xFv-hRIPK3
Plasmid#106079Purposeexpression in mammalian cellsDepositorAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nanog-2A-mCherry
Plasmid#48680PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanog (Nanog Mouse)
UseCRISPRTagsCherryAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VV073: 1xCox8 - GCaMP5G in fubi
Plasmid#58509PurposeExpresses the calcium sensor GCaMP5G in the mitochondriaDepositorInsert1xCox8 - GCaMP5G
UseLentiviralExpressionMammalianPromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
MuHKI-pGFPN3
Plasmid#21919DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK1 Human)
TagsGFPExpressionMammalianMutationThis is the full length human HKI cDNA sequence w…Available sinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaRlux
Plasmid#47802PurposePesaR promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorPublicationInsertPesaR-luxCDABE-term
UseSynthetic BiologyExpressionBacterialPromoterPesaRAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP-P2A_BAF_G47E
Plasmid#101774PurposeExpresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)DepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFP-P2AExpressionMammalianMutationG47E mutationPromoterEF1aAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pCAG-FLEX-mRuby3-WPRE
Plasmid#99279PurposeCan be used to generate AAV virus that will express mRuby3 in the presence of CreDepositorInsertmRuby3
UseAAV and Cre/LoxAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC-85b
Plasmid#53282PurposeContains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthaseDepositorInsertcrtE, crtY, crtI
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG::PSAML141F,Y115F:GlyR-IRES-GFP
Plasmid#32480PurposeExpresses PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPSAML141F,Y115F-GlyR
TagsIRES-GFPExpressionMammalianPromoterCAGAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBbB8k-csg-NbGFP
Plasmid#166858PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.DepositorInsertcsgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
UseSynthetic BiologyExpressionBacterialAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB6364
Plasmid#106151PurposeEasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistanceDepositorInsertCas9
ExpressionYeastAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by