We narrowed to 22,070 results for: ESC

1. pMRE164

   Plasmid

   #118512

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mOrange2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAD-SPS6-Pman

   Plasmid

   #133906

   Purpose

   Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(SPS6)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pman

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-CMV-TetO2_mTurquoise2

   Plasmid

   #113895

   Purpose

   Mammalian (inducible) expression of cytosolic mTurquoise2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Insert

   monomeric Turquoise2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Perox-B

   Plasmid

   #209867

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Use

   Tags

   Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-GCaMP3fast

   Plasmid

   #74422

   Purpose

   Express fluorescent calcium indicator GCaMP3fast in mammalian cells

   Depositor

   Katalin Torok

   Article

   Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

   Insert

   GCaMP3fast
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W4Y D356A

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CaMKIIa-Archer1-EGFP

   Plasmid

   #60423

   Purpose

   Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

   Depositor

   Viviana Gradinaru

   Article

   Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

   Insert

   Archer1-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b/bPanK

   Plasmid

   #73619

   Purpose

   Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

   Depositor

   Suzanne Jackowski, T. J. Kappock

   Article

   Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

   Insert

   pantothenate kinase (coaA Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   relative to MG1655 sequence, changes glutamate-5 …

   Promoter

   T7

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-CMV-TetO2_3C-Twin-Strep

   Plasmid

   #113883

   Purpose

   Mammalian (inducible) protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMW35 p21 (mouse)

   Plasmid

   #16478

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 (Cdkn1a Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pMito-MtrxRFP2

    Plasmid

    #172324

    Purpose

    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    

    Use

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-EBFP2-P2A-EGFP

    Plasmid

    #184938

    Purpose

    Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    EBFP2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-TDsmURFP-RBS-HO-1

    Plasmid

    #80342

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Use

    Tags

    6 Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRE141

    Plasmid

    #118493

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-syn-fRCaMP2

    Plasmid

    #125246

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    synapsin

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBW-m4046757

    Plasmid

    #145657

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAD-mKate(KPS12)

    Plasmid

    #133831

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pupp

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b_EcSSB_1

    Plasmid

    #78203

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

    Insert

    ssb (G26C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G26C (please see depositor comment below)

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71346

    Purpose

    Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-erHRP(N175S mutant)

    Plasmid

    #79909

    Purpose

    erHRP(N175S mutant) pCMV backbone

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    erHRP(N175S mutant)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    N175S mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD-HisD-KRaION2

    Plasmid

    #177800

    Purpose

    Bacterial expression of green fluorescent potassium indicator KRaION2

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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