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Plasmid#158732PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR6A L3-L2
Plasmid#158733PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NG)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR7A L3-L2
Plasmid#158734PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NI)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS4S
Plasmid#55170PurposeEmpty vector, integration at thrC, ampr, specrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1
Plasmid#166823PurposeGateway pDONR vector containing NLRP1 (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-E795L
Plasmid#176890PurposeGateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoterDepositorInsertLbCas12a-E795L
UseCRISPR; Gateway compatible lbcpf1 entry cloneTagsNucleoplasmin NLS and SV40 NLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAK184
Plasmid#167281PurposeMazF-based mutagenesis vector for C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterPxylAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS3Clux
Plasmid#55172Purposelux-reporter vector, integration at sacA, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10A, H840APromoterPj23119 and ptbAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-TRE-mCherry-attB53
Plasmid#183611PurposeRMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertTRE-mCherry
UseRmceTagsExpressionMutationPromoterTREAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS1ClacZ
Plasmid#55171PurposelacZ-reporter vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAD-SPS6-Pman
Plasmid#133906PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE657
Plasmid#153255PurposeM2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE479
Plasmid#153270PurposeM4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEG R3-miRNA(ccdB)-L4 #BGV228
Plasmid#48953PurposeTo create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST
Plasmid#203812PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD-mKate(KPS12)
Plasmid#133831PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPuppAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only