We narrowed to 5,610 results for: KRAS;

Showing: 2641 - 2660 of 5610 results

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pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3646

Plasmid

#137714

Purpose

Expresses LbCas12a RVRR variant in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

LbCas12a-RVRR

Tags

3xHA, NLS, and SV40 NLS

Expression

Mammalian

Mutation

G532R, K538V, Y542R, K595R

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR221-GLI1

Plasmid

#79497

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

GLI1 (GLI1 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-MAPTau-C-10

Plasmid

#55431

Purpose

Localization: Microtubules, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

MAPTau (MAPT Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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LRRK2-K1906M

Plasmid

#35946

Depositor

Zhaoyang Feng

Article

Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

Insert

LRRK2 (LRRK2 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-PLAA-Strep-HA

Plasmid

#113484

Purpose

Expression of human PLAA with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

PLAA (PLAA Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-HA-TEV

Plasmid

#105769

Purpose

Expression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

HA-TEV

Expression

Mammalian

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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AA290

Plasmid

#215949

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD55_v4; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA286

Plasmid

#215948

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgCD47_v6; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-HA

Plasmid

#105785

Purpose

Expression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-HA

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4938

Plasmid

#107527

Purpose

Expresses Fn crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75232

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR221-CCL5.Pro

Plasmid

#79492

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CCL5 Promoter (CCL5 Synthetic, Human)

Use

Multisite gateway entry clone

Promoter

CCL5 Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-mTDGa.1

Plasmid

#81051

Purpose

bacterial expression of catalytically dead murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

asparagine 151 changed to alanine

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-YOD1-Strep-HA

Plasmid

#113483

Purpose

Expression of human YOD1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

YOD1 (YOD1 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAT15546_nABE8e-SuperFi-Cas9

Plasmid

#184376

Purpose

Mammalian expression of SuperFi-Cas9 ABE8e base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nABE8e-SuperFi-Cas9

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTE4560

Plasmid

#107526

Purpose

Expresses As crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

As crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-miR-125b-1

Plasmid

#58990

Purpose

MSCV based expression vector - co-expresses mCherry and human miR-125b-1

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Inserts

mCherry fluorescent protein
miR-125b-1 (MIR125B1 Human)

Use

Retroviral; Mscv-based retroviral system

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Integrin-Beta1-N-18

Plasmid

#55423

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

Plasmid

#124870

Purpose

Mutagenesis of Gabrg1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg1 (Gabrg1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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